biostudies-literatureUnknown8Kim BSNational Research Foundation of KoreaSeoul National UniversityPolyphenols can trigger immunity that activates intracellular anti-inflammatory signaling and prevents external infections. In this study, we report the fabrication of chitosan-based hydrogels with epigallocatechin gallate (EGCG) using enzyme-mediated one-pot synthesis. The tyrosinase-mediated oxidative reaction of the phenolic rings of EGCG with the primary amines on chitosan results in stable EGCG-chitosan hydrogels. The EGCG concentrations contributed to the cross-linking density and physical properties of EGCG-chitosan hydrogels. Furthermore, EGCG-chitosan hydrogels maintained intrinsic properties such as antibacterial and antioxidant effects. When endotoxin-activated RAW 264.7 macrophage cells were cultured with EGCG-chitosan hydrogels, the hydrogels reduced the inflammatory response Materials today. Bio100079https://www.ebi.ac.uk/biostudies/studies/S-EPMC7575804biostudies-literatureEnzyme-mediated one-pot synthesis of hydrogel with the polyphenol cross-linker for skin regeneration.PMC7575804Kim SHKim BGAn YHSo KHKim BSHwang NSKim KfalseEnzyme-mediated one-pot synthesis of hydrogel with the polyphenol cross-linker for skin regeneration.Polyphenols can trigger immunity that activates intracellular anti-inflammatory signaling and prevents external infections. In this study, we report the fabrication of chitosan-based hydrogels with epigallocatechin gallate (EGCG) using enzyme-mediated one-pot synthesis. The tyrosinase-mediated oxidative reaction of the phenolic rings of EGCG with the primary amines on chitosan results in stable EGCG-chitosan hydrogels. The EGCG concentrations contributed to the cross-linking density and physical properties of EGCG-chitosan hydrogels. Furthermore, EGCG-chitosan hydrogels maintained intrinsic properties such as antibacterial and antioxidant effects. When endotoxin-activated RAW 264.7 macrophage cells were cultured with EGCG-chitosan hydrogels, the hydrogels reduced the inflammatory response 2020-01-01T00:00:00Z2020 Sep2026-05-07T19:23:11.159Z2026-04-07T23:13:32.568ZS-EPMC75758043310310510.1016/j.mtbio.2020.100079