{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(17)"],"submitter":["Jin W"],"pubmed_abstract":["<h4>Background</h4>To evaluate the diagnostic accuracy of metagenomic next-generation sequencing (mNGS) for active tuberculosis (TB).<h4>Methods</h4>We retrospectively collected 820 samples at Zhongshan Hospital, Fudan University in Shanghai, China, between 1 April 2017 and 31 March 2018. They were classified into TB cases (125, 15.2%) and NOT TB cases (695, 84.8%) according to the clinical diagnosis. Specimens were evaluated by a regular clinical microbiological assay and mNGS performed in parallel.<h4>Results</h4>Sixty-one confirmed TB cases and 64 clinical TB cases were included. The overall sensitivity of mNGS was 49.6% [95% confidence interval (95% CI), 40.6-58.6%], and the specificity was 98.3% (95% CI, 96.9-99.1%), with peak sensitivities of 88.9% (95% CI, 50.7-99.4%) for lung tissu"],"journal":["Annals of translational medicine"],"pagination":["1065"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7575944"],"repository":["biostudies-literature"],"pubmed_title":["Diagnostic accuracy of metagenomic next-generation sequencing for active tuberculosis in clinical practice at a tertiary general hospital."],"pmcid":["PMC7575944"],"pubmed_authors":["Miao Q","Jin W","Ma Y","Bao R","Hu B","Wu H","Wang M","Pan J","Yao Y","Huang Y","Wang Q","Zhang Y","Li B","Gao X","Su Y"],"additional_accession":[]},"is_claimable":false,"name":"Diagnostic accuracy of metagenomic next-generation sequencing for active tuberculosis in clinical practice at a tertiary general hospital.","description":"<h4>Background</h4>To evaluate the diagnostic accuracy of metagenomic next-generation sequencing (mNGS) for active tuberculosis (TB).<h4>Methods</h4>We retrospectively collected 820 samples at Zhongshan Hospital, Fudan University in Shanghai, China, between 1 April 2017 and 31 March 2018. They were classified into TB cases (125, 15.2%) and NOT TB cases (695, 84.8%) according to the clinical diagnosis. Specimens were evaluated by a regular clinical microbiological assay and mNGS performed in parallel.<h4>Results</h4>Sixty-one confirmed TB cases and 64 clinical TB cases were included. The overall sensitivity of mNGS was 49.6% [95% confidence interval (95% CI), 40.6-58.6%], and the specificity was 98.3% (95% CI, 96.9-99.1%), with peak sensitivities of 88.9% (95% CI, 50.7-99.4%) for lung tissu","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2025-04-19T23:38:59.38Z","creation":"2025-04-19T23:38:59.38Z"},"accession":"S-EPMC7575944","cross_references":{"pubmed":["33145284"],"doi":["10.21037/atm-20-2274"]}}