biostudies-literatureMa QJoint Programs of Henan Provincial Government and Healthy MinistryHenan Provincial Key Programs in Science and Technology318https://www.ebi.ac.uk/biostudies/studies/S-EPMC7576717biostudies-literatureUnknown20(1)<h4>Background</h4>To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2342 isolates were obtained from microbial cultures of clinical specimens (e.g. blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People's Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Inconsistencies in strain identification among the three systems were identified by 16S rDNA and gene sequencing.<h4>Results</h4>At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identiBMC microbiologyEvaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates.PMC7576717SB2018084 SB20190318 LHGJ20190611181200211900Ma QLi YZhang JWang SWang YZhang QYan WXu JYuan YfalseEvaluation of the Autof MS1000 mass spectrometer in the identification of clinical isolates.<h4>Background</h4>To evaluate the accuracy and performance of the Autof MS1000 mass spectrometer in bacteria and yeast identification, 2342 isolates were obtained from microbial cultures of clinical specimens (e.g. blood, cerebrospinal fluid, respiratory tract samples, lumbar puncture fluid, wound samples, stool, and urine) collected in 2019 in Henan Provincial People's Hospital. Repetitive strains from the same patient were excluded. We tested the Autof MS1000 and Bruker Biotyper mass spectrometry systems and the classical biochemical identification system VITEK 2/API 20C AUX. Inconsistencies in strain identification among the three systems were identified by 16S rDNA and gene sequencing.<h4>Results</h4>At the species level, the Autof MS1000 and Bruker Biotyper systems had isolate identi2020-01-01T00:00:00Z2020 Oct2025-04-26T13:35:52.552Z2020-10-29T14:50:00ZS-EPMC75767173308172210.1186/s12866-020-02005-0