{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Cao Chu U"],"pubmed_abstract":["<i>Agrobacterium</i>-mediated transformation of canola (<i>Brassica napus</i>) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an <i>Agrobacterium</i>-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as"],"journal":["Frontiers in plant science"],"pagination":["579524"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7578431"],"repository":["biostudies-literature"],"pubmed_title":["Genotype-Independent Transformation and Genome Editing of <i>Brassica napus</i> Using a Novel Explant Material."],"pmcid":["PMC7578431"],"pubmed_authors":["Sigmund A","Johnson K","Li Y","Cao Chu U","Vongdeuane P","Jones TJ","Kumar S"],"additional_accession":[]},"is_claimable":false,"name":"Genotype-Independent Transformation and Genome Editing of <i>Brassica napus</i> Using a Novel Explant Material.","description":"<i>Agrobacterium</i>-mediated transformation of canola (<i>Brassica napus</i>) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an <i>Agrobacterium</i>-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-19T23:40:46.252Z","creation":"2020-11-03T08:04:52Z"},"accession":"S-EPMC7578431","cross_references":{"pubmed":["33133118"],"doi":["10.3389/fpls.2020.579524"]}}