biostudies-literatureUnknown11Cao Chu U<i>Agrobacterium</i>-mediated transformation of canola (<i>Brassica napus</i>) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an <i>Agrobacterium</i>-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well asFrontiers in plant science579524https://www.ebi.ac.uk/biostudies/studies/S-EPMC7578431biostudies-literatureGenotype-Independent Transformation and Genome Editing of <i>Brassica napus</i> Using a Novel Explant Material.PMC7578431Sigmund AJohnson KLi YCao Chu UVongdeuane PJones TJKumar SfalseGenotype-Independent Transformation and Genome Editing of <i>Brassica napus</i> Using a Novel Explant Material.<i>Agrobacterium</i>-mediated transformation of canola (<i>Brassica napus</i>) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an <i>Agrobacterium</i>-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as2020-01-01T00:00:00Z20202025-04-19T23:40:46.252Z2020-11-03T08:04:52ZS-EPMC75784313313311810.3389/fpls.2020.579524