{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lai TC"],"funding":["Ministry of Science and Technology, Taiwan","Academia Sinica"],"pagination":["164"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7579951"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(1)"],"pubmed_abstract":["<h4>Background</h4>Chemotherapy is currently one of the most effective treatments for advanced breast cancer. Anti-microtubule agents, including taxanes, eribulin and vinca-alkaloids are one of the primary major anti-breast cancer chemotherapies; however, chemoresistance remains a problem that is difficult to solve. We aimed to discover novel candidate protein targets to combat chemoresistance in breast cancer.<h4>Methods</h4>A lentiviral shRNA-based high-throughput screening platform was designed and developed to screen the global kinome to find new therapeutic targets in paclitaxel-resistant breast cancer cells. The phenotypes were confirmed with alternative expression in vitro and in vivo. Molecular mechanisms were investigated using global phosphoprotein arrays and expression microarra"],"journal":["Cell communication and signaling : CCS"],"pubmed_title":["Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway."],"pmcid":["PMC7579951"],"funding_grant_id":["MOST 106-0210-01-15-02, MOST 107-0210-01-19-01"],"pubmed_authors":["Yang YF","Chang PM","Liu CY","Fang CY","Jan YH","Hsiao M","Lai TC","Hsieh HL"],"additional_accession":[]},"is_claimable":false,"name":"Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.","description":"<h4>Background</h4>Chemotherapy is currently one of the most effective treatments for advanced breast cancer. Anti-microtubule agents, including taxanes, eribulin and vinca-alkaloids are one of the primary major anti-breast cancer chemotherapies; however, chemoresistance remains a problem that is difficult to solve. We aimed to discover novel candidate protein targets to combat chemoresistance in breast cancer.<h4>Methods</h4>A lentiviral shRNA-based high-throughput screening platform was designed and developed to screen the global kinome to find new therapeutic targets in paclitaxel-resistant breast cancer cells. The phenotypes were confirmed with alternative expression in vitro and in vivo. Molecular mechanisms were investigated using global phosphoprotein arrays and expression microarra","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-07-16T07:09:57.033Z","creation":"2020-10-29T15:57:56Z"},"accession":"S-EPMC7579951","cross_references":{"pubmed":["33087151"],"doi":["10.1186/s12964-020-00600-2"]}}