biostudies-literatureLai TCMinistry of Science and Technology, TaiwanAcademia Sinica164https://www.ebi.ac.uk/biostudies/studies/S-EPMC7579951biostudies-literatureUnknown18(1)<h4>Background</h4>Chemotherapy is currently one of the most effective treatments for advanced breast cancer. Anti-microtubule agents, including taxanes, eribulin and vinca-alkaloids are one of the primary major anti-breast cancer chemotherapies; however, chemoresistance remains a problem that is difficult to solve. We aimed to discover novel candidate protein targets to combat chemoresistance in breast cancer.<h4>Methods</h4>A lentiviral shRNA-based high-throughput screening platform was designed and developed to screen the global kinome to find new therapeutic targets in paclitaxel-resistant breast cancer cells. The phenotypes were confirmed with alternative expression in vitro and in vivo. Molecular mechanisms were investigated using global phosphoprotein arrays and expression microarraCell communication and signaling : CCSKinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.PMC7579951MOST 106-0210-01-15-02, MOST 107-0210-01-19-01Yang YFChang PMLiu CYFang CYJan YHHsiao MLai TCHsieh HLfalseKinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.<h4>Background</h4>Chemotherapy is currently one of the most effective treatments for advanced breast cancer. Anti-microtubule agents, including taxanes, eribulin and vinca-alkaloids are one of the primary major anti-breast cancer chemotherapies; however, chemoresistance remains a problem that is difficult to solve. We aimed to discover novel candidate protein targets to combat chemoresistance in breast cancer.<h4>Methods</h4>A lentiviral shRNA-based high-throughput screening platform was designed and developed to screen the global kinome to find new therapeutic targets in paclitaxel-resistant breast cancer cells. The phenotypes were confirmed with alternative expression in vitro and in vivo. Molecular mechanisms were investigated using global phosphoprotein arrays and expression microarra2020-01-01T00:00:00Z2020 Oct2026-07-16T07:09:57.033Z2020-10-29T15:57:56ZS-EPMC75799513308715110.1186/s12964-020-00600-2