{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tsai CN"],"funding":["Boris Family Fund for Health Research Excellence","Canadian Institutes of Health Research","CIHR"],"pagination":["100057"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7580248"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1(2)"],"pubmed_abstract":["Anti-virulence therapies are under active investigation as antibiotic alternatives; however, their identification from large-scale chemical libraries poses a unique challenge. The dispensability of virulence factors for growth <i>in vitro</i> precludes conventional, optical density-based screening methods. Here, we provide a protocol for high-throughput screening with a cell-based, promoter reporter platform. We describe the use of this method for the identification of anti-SPI-2 inhibitors specific to <i>Salmonella</i> Typhimurium, which may be modified to investigate other virulence factors. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2020)."],"journal":["STAR protocols"],"pubmed_title":["High-Throughput Chemical Screening for Inhibitors of <i>Salmonella</i> Pathogenicity Island 2."],"pmcid":["PMC7580248"],"funding_grant_id":["FRN-156361","FRN-15636"],"pubmed_authors":["Tsai CN","Coombes BK"],"additional_accession":[]},"is_claimable":false,"name":"High-Throughput Chemical Screening for Inhibitors of <i>Salmonella</i> Pathogenicity Island 2.","description":"Anti-virulence therapies are under active investigation as antibiotic alternatives; however, their identification from large-scale chemical libraries poses a unique challenge. The dispensability of virulence factors for growth <i>in vitro</i> precludes conventional, optical density-based screening methods. Here, we provide a protocol for high-throughput screening with a cell-based, promoter reporter platform. We describe the use of this method for the identification of anti-SPI-2 inhibitors specific to <i>Salmonella</i> Typhimurium, which may be modified to investigate other virulence factors. For complete details on the use and execution of this protocol, please refer to Tsai et al. (2020).","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Sep","modification":"2025-05-29T19:38:10.003Z","creation":"2025-05-29T19:38:10.003Z"},"accession":"S-EPMC7580248","cross_references":{"pubmed":["33111100"],"doi":["10.1016/j.xpro.2020.100057"]}}