biostudies-literatureOverby HNIDDK NIH HHSNIEHS NIH HHSNational Institutes of HealthNIH HHSE7039https://www.ebi.ac.uk/biostudies/studies/S-EPMC7582898biostudies-literatureUnknown21(19)Brown adipose tissue (BAT) is an important target for obesity treatment and prevention. Soluble epoxide hydrolase (sEH) converts bioactive epoxy fatty acids (EpFAs) into less active diols. sEH inhibitors (sEHI) are beneficial in many chronic diseases by stabilizing EpFAs. However, roles of sEH and sEHI in brown adipogenesis and BAT activity in treating diet-induced obesity (DIO) have not been reported. sEH expression was studied in in vitro models of brown adipogenesis and the fat tissues of DIO mice. The effects of the sEHI, <i>trans</i>-4-{4-[3-(4-trifluoromethoxy-phenyl)-ureido]-cyclohexyloxy-benzoic acid (<i>t</i>-TUCB), were studied in vitro and in the obese mice via mini osmotic pump delivery. sEH expression was increased in brown adipogenesis and the BAT of the DIO mice. <i>t</i>-TUInternational journal of molecular sciencesSoluble Epoxide Hydrolase Inhibition by <i>t</i>-TUCB Promotes Brown Adipogenesis and Reduces Serum Triglycerides in Diet-Induced Obesity.PMC75828981R15DK114790-01A1R15 DK114790P42ES004699R15AT008733R00DK100736R35ES030443P42 ES004699Graham KBettaieb AHammock BDChoi SZeldin DCYang YZhao LWang SOverby HXu XHildreth KMorisseau CWan DfalseSoluble Epoxide Hydrolase Inhibition by <i>t</i>-TUCB Promotes Brown Adipogenesis and Reduces Serum Triglycerides in Diet-Induced Obesity.Brown adipose tissue (BAT) is an important target for obesity treatment and prevention. Soluble epoxide hydrolase (sEH) converts bioactive epoxy fatty acids (EpFAs) into less active diols. sEH inhibitors (sEHI) are beneficial in many chronic diseases by stabilizing EpFAs. However, roles of sEH and sEHI in brown adipogenesis and BAT activity in treating diet-induced obesity (DIO) have not been reported. sEH expression was studied in in vitro models of brown adipogenesis and the fat tissues of DIO mice. The effects of the sEHI, <i>trans</i>-4-{4-[3-(4-trifluoromethoxy-phenyl)-ureido]-cyclohexyloxy-benzoic acid (<i>t</i>-TUCB), were studied in vitro and in the obese mice via mini osmotic pump delivery. sEH expression was increased in brown adipogenesis and the BAT of the DIO mice. <i>t</i>-TU2020-01-01T00:00:00Z2020 Sep2025-04-21T20:36:56.738Z2020-10-31T09:57:52ZS-EPMC75828983298788010.3390/ijms21197039