{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suleiman E"],"funding":["Austrian Science Fund FWF","Horizon 2020 Framework Programme"],"pagination":["E979"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7589475"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(10)"],"pubmed_abstract":["The display of native-like human immunodeficiency virus type 1 envelope (HIV-1 Env) trimers on liposomes has gained wide attention over the last few years. Currently, available methods have enabled the preparation of Env-liposome conjugates of unprecedented quality. However, these protocols require the Env trimer to be tagged and/or to carry a specific functional group. For this reason, we have investigated <i>N</i>-(3-Dimethylaminopropyl)-<i>N</i>'-ethylcarbodiimide/<i>N</i>-Hydroxysulfosuccinimide (EDC/Sulfo-NHS) chemistry for its potential to covalently conjugate tag-free, non-functionalized native-like Env trimers onto the surface of carboxyl-functionalized liposomes. The preservation of the liposome's physical integrity and the immunogen's conformation required a fine-tuned two-step a"],"journal":["Pharmaceutics"],"pubmed_title":["Conjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations."],"pmcid":["PMC7589475"],"funding_grant_id":["FWF W1224","W 1224","681137"],"pubmed_authors":["Mayer J","Damm D","Uberla K","Vorauer-Uhl K","Suleiman E","Lehner E","Kohlhauser B","Temchura V","Katholnig A","Batzoni M","Wagner A"],"additional_accession":[]},"is_claimable":false,"name":"Conjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations.","description":"The display of native-like human immunodeficiency virus type 1 envelope (HIV-1 Env) trimers on liposomes has gained wide attention over the last few years. Currently, available methods have enabled the preparation of Env-liposome conjugates of unprecedented quality. However, these protocols require the Env trimer to be tagged and/or to carry a specific functional group. For this reason, we have investigated <i>N</i>-(3-Dimethylaminopropyl)-<i>N</i>'-ethylcarbodiimide/<i>N</i>-Hydroxysulfosuccinimide (EDC/Sulfo-NHS) chemistry for its potential to covalently conjugate tag-free, non-functionalized native-like Env trimers onto the surface of carboxyl-functionalized liposomes. The preservation of the liposome's physical integrity and the immunogen's conformation required a fine-tuned two-step a","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-22T18:53:22.297Z","creation":"2020-11-01T08:24:52Z"},"accession":"S-EPMC7589475","cross_references":{"pubmed":["33081278"],"doi":["10.3390/pharmaceutics12100979"]}}