biostudies-literatureSuleiman EAustrian Science Fund FWFHorizon 2020 Framework ProgrammeE979https://www.ebi.ac.uk/biostudies/studies/S-EPMC7589475biostudies-literatureUnknown12(10)The display of native-like human immunodeficiency virus type 1 envelope (HIV-1 Env) trimers on liposomes has gained wide attention over the last few years. Currently, available methods have enabled the preparation of Env-liposome conjugates of unprecedented quality. However, these protocols require the Env trimer to be tagged and/or to carry a specific functional group. For this reason, we have investigated <i>N</i>-(3-Dimethylaminopropyl)-<i>N</i>'-ethylcarbodiimide/<i>N</i>-Hydroxysulfosuccinimide (EDC/Sulfo-NHS) chemistry for its potential to covalently conjugate tag-free, non-functionalized native-like Env trimers onto the surface of carboxyl-functionalized liposomes. The preservation of the liposome's physical integrity and the immunogen's conformation required a fine-tuned two-step aPharmaceuticsConjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations.PMC7589475FWF W1224W 1224681137Mayer JDamm DUberla KVorauer-Uhl KSuleiman ELehner EKohlhauser BTemchura VKatholnig ABatzoni MWagner AfalseConjugation of Native-Like HIV-1 Envelope Trimers onto Liposomes Using EDC/Sulfo-NHS Chemistry: Requirements and Limitations.The display of native-like human immunodeficiency virus type 1 envelope (HIV-1 Env) trimers on liposomes has gained wide attention over the last few years. Currently, available methods have enabled the preparation of Env-liposome conjugates of unprecedented quality. However, these protocols require the Env trimer to be tagged and/or to carry a specific functional group. For this reason, we have investigated <i>N</i>-(3-Dimethylaminopropyl)-<i>N</i>'-ethylcarbodiimide/<i>N</i>-Hydroxysulfosuccinimide (EDC/Sulfo-NHS) chemistry for its potential to covalently conjugate tag-free, non-functionalized native-like Env trimers onto the surface of carboxyl-functionalized liposomes. The preservation of the liposome's physical integrity and the immunogen's conformation required a fine-tuned two-step a2020-01-01T00:00:00Z2020 Oct2025-04-22T18:53:22.297Z2020-11-01T08:24:52ZS-EPMC75894753308127810.3390/pharmaceutics12100979