biostudies-literatureIsnaldi ECancer Foundation179-186https://www.ebi.ac.uk/biostudies/studies/S-EPMC7589564biostudies-literatureUnknown54Adipocytes and cancer-associated adipocytes (CAAs) are poorly investigated cells in the tumor microenvironment. Different image analysis software exist for identifying and measuring these cells using scanned hematoxylin and eosin (H&E)-stained slides. It is however unclear which one is the most appropriate for breast cancer (BC) samples. Here, we compared three software (AdipoCount, Adiposoft, and HALO®). HALO® outperformed the other methods with regard to adipocyte identification, (> 96% sensitivity and specificity). All software performed equally good with regard to area and diameter measurement (concordance correlation coefficients > 0.97 and > 0.96, respectively). We then analyzed a series of 10 BCE samples (n = 51 H&E slides) with HALO®. Distant adipocytes were defined >2 mm away fromBreast (Edinburgh, Scotland)Digital analysis of distant and cancer-associated mammary adipocytes.PMC7589564FAF-C/2018/1302FC/2018/07Maetens MIsnaldi ELeduc SSotiriou CZoppoli GLarsimont DRichard FGeukens TDe Schepper MFloris GDesmedt CRouas GCardoso FVincent DBiganzoli EfalseDigital analysis of distant and cancer-associated mammary adipocytes.Adipocytes and cancer-associated adipocytes (CAAs) are poorly investigated cells in the tumor microenvironment. Different image analysis software exist for identifying and measuring these cells using scanned hematoxylin and eosin (H&E)-stained slides. It is however unclear which one is the most appropriate for breast cancer (BC) samples. Here, we compared three software (AdipoCount, Adiposoft, and HALO®). HALO® outperformed the other methods with regard to adipocyte identification, (> 96% sensitivity and specificity). All software performed equally good with regard to area and diameter measurement (concordance correlation coefficients > 0.97 and > 0.96, respectively). We then analyzed a series of 10 BCE samples (n = 51 H&E slides) with HALO®. Distant adipocytes were defined >2 mm away from2020-01-01T00:00:00Z2020 Dec2025-04-18T21:35:15.908Z2020-11-03T08:04:15ZS-EPMC75895643312008310.1016/j.breast.2020.10.004