{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Newberg J"],"funding":["NICHD NIH HHS","NIA NIH HHS","National Institutes of Health","NIGMS NIH HHS","National Institute on Aging","National Science Foundation"],"pagination":["110012"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7590198"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["111"],"pubmed_abstract":["The nucleus, central to all cellular activity, relies on both direct mechanical input and its molecular transducers to sense and respond to external stimuli. While it has been shown that isolated nuclei can adapt to applied force ex vivo, the mechanisms governing nuclear mechanoadaptation in response to physiologic forces in vivo remain unclear. To investigate nuclear mechanoadaptation in cells, we developed an atomic force microscopy (AFM) based procedure to probe live nuclei isolated from mesenchymal stem cells (MSCs) following the application of low intensity vibration (LIV) to determine whether nuclear stiffness increases as a result of LIV. Results indicated that isolated nuclei were, on average, 30% softer than nuclei tested within intact MSCs prior to LIV. When the nucleus was isola"],"journal":["Journal of biomechanics"],"pubmed_title":["Isolated nuclei stiffen in response to low intensity vibration."],"pmcid":["PMC7590198"],"funding_grant_id":["R01 AG059923","P20 GM109095","P2C HD086843","P20 GM103408"],"pubmed_authors":["Schimpf J","Davis PH","Woods K","Loisate S","Newberg J","Uzer G"],"additional_accession":[]},"is_claimable":false,"name":"Isolated nuclei stiffen in response to low intensity vibration.","description":"The nucleus, central to all cellular activity, relies on both direct mechanical input and its molecular transducers to sense and respond to external stimuli. While it has been shown that isolated nuclei can adapt to applied force ex vivo, the mechanisms governing nuclear mechanoadaptation in response to physiologic forces in vivo remain unclear. To investigate nuclear mechanoadaptation in cells, we developed an atomic force microscopy (AFM) based procedure to probe live nuclei isolated from mesenchymal stem cells (MSCs) following the application of low intensity vibration (LIV) to determine whether nuclear stiffness increases as a result of LIV. Results indicated that isolated nuclei were, on average, 30% softer than nuclei tested within intact MSCs prior to LIV. When the nucleus was isola","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2026-05-07T12:10:33.297Z","creation":"2022-02-11T11:55:35.862Z"},"accession":"S-EPMC7590198","cross_references":{"pubmed":["32932075"],"doi":["10.1016/j.jbiomech.2020.110012"]}}