biostudies-literatureUnknown11Shen-Gunther JBrooke Army Medical CenterPrimary high-risk Human Papillomavirus (hrHPV) screening has recently become an accepted standalone or co-test with conventional cytology. Unfortunately, hrHPV singularly lacks specificity for cytopathological grade. However, mechanisms and markers of evolving virus-host interactions at the epigenome level may be harnessed as a better predictor of carcinogenesis. This study aimed to validate and expand the clinical performance of a multiparametric biomarker panel, referred to as the "Molecular Pap smear" based, on HPV genotype and <i>ADCY8</i>, <i>CDH8</i> and <i>ZNF582</i> CpG-methylation as a predictive classifier of cervical cytology. This prospective, cross-sectional study used an independent cohort of residual liquid-based cytology for HPV genotyping and epigenetic analysis. ExtractedFrontiers in microbiology595902https://www.ebi.ac.uk/biostudies/studies/S-EPMC7593258biostudies-literatureMolecular Pap Smear: Validation of HPV Genotype and Host Methylation Profiles of <i>ADCY8</i>, <i>CDH8</i>, and <i>ZNF582</i> as a Predictor of Cervical Cytopathology.PMC7593258Asusta HBStacey WShen-Gunther JXia QfalseMolecular Pap Smear: Validation of HPV Genotype and Host Methylation Profiles of <i>ADCY8</i>, <i>CDH8</i>, and <i>ZNF582</i> as a Predictor of Cervical Cytopathology.Primary high-risk Human Papillomavirus (hrHPV) screening has recently become an accepted standalone or co-test with conventional cytology. Unfortunately, hrHPV singularly lacks specificity for cytopathological grade. However, mechanisms and markers of evolving virus-host interactions at the epigenome level may be harnessed as a better predictor of carcinogenesis. This study aimed to validate and expand the clinical performance of a multiparametric biomarker panel, referred to as the "Molecular Pap smear" based, on HPV genotype and <i>ADCY8</i>, <i>CDH8</i> and <i>ZNF582</i> CpG-methylation as a predictive classifier of cervical cytology. This prospective, cross-sectional study used an independent cohort of residual liquid-based cytology for HPV genotyping and epigenetic analysis. Extracted2020-01-01T00:00:00Z20202026-07-09T11:19:36.07Z2020-11-19T11:39:22ZS-EPMC75932583317817510.3389/fmicb.2020.595902