{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Z"],"funding":["Medical Research Council","Royal Society of Biology","Wellcome Trust"],"pagination":["jcs246637"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7595685"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["133(20)"],"pubmed_abstract":["Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the <i>Leishmania</i> parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat to rotate the cell. In multi-ciliated tissues or organisms, the asymmetric beats are coordinated, leading to movement of the cell, organism or surrounding fluid. This coordination involves a polarisation of power stroke direction. Here, we asked whether the asymmetric beat of the single <i>Leishmania</i> flagellum also has a fixed polarisation. We developed high frame rate dual-colour fluorescence microscopy to visualise flagellar-associated structures in live swimming cells. This showed that the asymmetric <i>Leishmania</i> beat is polarised, with power s"],"journal":["Journal of cell science"],"pubmed_title":["The single flagellum of <i>Leishmania</i> has a fixed polarisation of its asymmetric beat."],"pmcid":["PMC7595685"],"funding_grant_id":["211075/Z/18/Z","UF160661","104627/Z/14/Z","15/16_MSD_836338","1643029","UF100435 and"],"pubmed_authors":["Beneke T","Gluenz E","Wang Z","Wheeler RJ"],"additional_accession":[]},"is_claimable":false,"name":"The single flagellum of <i>Leishmania</i> has a fixed polarisation of its asymmetric beat.","description":"Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the <i>Leishmania</i> parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat to rotate the cell. In multi-ciliated tissues or organisms, the asymmetric beats are coordinated, leading to movement of the cell, organism or surrounding fluid. This coordination involves a polarisation of power stroke direction. Here, we asked whether the asymmetric beat of the single <i>Leishmania</i> flagellum also has a fixed polarisation. We developed high frame rate dual-colour fluorescence microscopy to visualise flagellar-associated structures in live swimming cells. This showed that the asymmetric <i>Leishmania</i> beat is polarised, with power s","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-22T16:49:57.99Z","creation":"2020-11-08T09:46:28Z"},"accession":"S-EPMC7595685","cross_references":{"pubmed":["33093230"],"doi":["10.1242/jcs.246637"]}}