biostudies-literatureKu JMinistry of Science, ICT and Future PlanningThis research was also supported with the computing resources by Global Science experimental Data hub Center (GSDC) & Korea Research Environment Open NETwork (KREONET).630https://www.ebi.ac.uk/biostudies/studies/S-EPMC7599330biostudies-literatureUnknown3(1)Aminoacyl-tRNA synthetase-interacting multifunctional protein 2 (AIMP2) is a non-enzymatic component required for the multi-tRNA synthetase complex. While exon 2 skipping alternatively spliced variant of AIMP2 (AIMP2-DX2) compromises AIMP2 activity and is associated with carcinogenesis, its clinical potential awaits further validation. Here, we found that AIMP2-DX2/AIMP2 expression ratio is strongly correlated with major cancer signaling pathways and poor prognosis, particularly in acute myeloid leukemia (AML). Analysis of a clinical patient cohort revealed that AIMP2-DX2 positive AML patients show decreased overall survival and progression-free survival. We also developed targeted RNA-sequencing and single-molecule RNA-FISH tools to quantitatively analyze AIMP2-DX2/AIMP2 ratios at the sinCommunications biologySingle-cell analysis of AIMP2 splice variants informs on drug sensitivity and prognosis in hematologic cancer.PMC7599330NRF-2014M3A6A4074727NRF-2019R1C1C1006672Kim SKim DKim RLee NKu JYoon SSSong SLee KKoh YKim YKwon NHKim MfalseSingle-cell analysis of AIMP2 splice variants informs on drug sensitivity and prognosis in hematologic cancer.Aminoacyl-tRNA synthetase-interacting multifunctional protein 2 (AIMP2) is a non-enzymatic component required for the multi-tRNA synthetase complex. While exon 2 skipping alternatively spliced variant of AIMP2 (AIMP2-DX2) compromises AIMP2 activity and is associated with carcinogenesis, its clinical potential awaits further validation. Here, we found that AIMP2-DX2/AIMP2 expression ratio is strongly correlated with major cancer signaling pathways and poor prognosis, particularly in acute myeloid leukemia (AML). Analysis of a clinical patient cohort revealed that AIMP2-DX2 positive AML patients show decreased overall survival and progression-free survival. We also developed targeted RNA-sequencing and single-molecule RNA-FISH tools to quantitatively analyze AIMP2-DX2/AIMP2 ratios at the sin2020-01-01T00:00:00Z2020 Oct2025-06-01T03:53:32.805Z2025-06-01T03:53:32.805ZS-EPMC75993303312801410.1038/s42003-020-01353-x