biostudies-literatureEmri EEYE-RISK project funded by European Union's Horizon 2020 research and innovation programmeF. Hoffmann-La RocheEYE-RISK project funded by European Union's Horizon 2020 research and innovation programmeWellcome TrustE3051https://www.ebi.ac.uk/biostudies/studies/S-EPMC7601425biostudies-literatureUnknown12(10)In age-related macular degeneration (AMD), both systemic and local zinc levels decline. Elevation of zinc in clinical studies delayed the progression to end-stage AMD. However, the molecular pathways underpinning this beneficial effect are not yet identified. In this study, we used differentiated primary human fetal retinal pigment epithelium (RPE) cultures and long-term zinc supplementation to carry out a combined transcriptome, proteome and secretome analysis from three genetically different human donors. After combining significant differences, we identified the complex molecular networks using Database for Annotation, Visualization and Integrated Discovery (DAVID) and Ingenuity Pathway Analysis (IPA). The cell cultures from the three donors showed extensive pigmentation, development ofNutrientsA Multi-Omics Approach Identifies Key Regulatory Pathways Induced by Long-Term Zinc Supplementation in Human Primary Retinal Pigment Epithelium.PMC7601425634479unrestricted postdoctoral fellowshipKortvely EDen Hollander AIConsortium ERKlose FEmri ESimpson DLengyel IDammeier SUeffing MfalseA Multi-Omics Approach Identifies Key Regulatory Pathways Induced by Long-Term Zinc Supplementation in Human Primary Retinal Pigment Epithelium.In age-related macular degeneration (AMD), both systemic and local zinc levels decline. Elevation of zinc in clinical studies delayed the progression to end-stage AMD. However, the molecular pathways underpinning this beneficial effect are not yet identified. In this study, we used differentiated primary human fetal retinal pigment epithelium (RPE) cultures and long-term zinc supplementation to carry out a combined transcriptome, proteome and secretome analysis from three genetically different human donors. After combining significant differences, we identified the complex molecular networks using Database for Annotation, Visualization and Integrated Discovery (DAVID) and Ingenuity Pathway Analysis (IPA). The cell cultures from the three donors showed extensive pigmentation, development of2020-01-01T00:00:00Z2020 Oct2026-05-01T01:25:21.64Z2020-11-03T08:02:49ZS-EPMC76014253303619710.3390/nu12103051