biostudies-literatureFernandez VSpanish State Research AgencyEuropean Research CouncilFundación Tatiana Pérez de Guzmán el Bueno (Tatiana Pérez Foundation of Guzmán el Bueno)Fundación Tatiana Pérez de Guzmán el Buenoe105479https://www.ebi.ac.uk/biostudies/studies/S-EPMC7604626biostudies-literatureUnknown39(21)Structural integrity and cellular homeostasis of the embryonic stem cell niche are critical for normal tissue development. In the telencephalic neuroepithelium, this is controlled in part by cell adhesion molecules and regulators of progenitor cell lineage, but the specific orchestration of these processes remains unknown. Here, we studied the role of microRNAs in the embryonic telencephalon as key regulators of gene expression. By using the early recombiner Rx-Cre mouse, we identify novel and critical roles of miRNAs in early brain development, demonstrating they are essential to preserve the cellular homeostasis and structural integrity of the telencephalic neuroepithelium. We show that Rx-Cre;Dicer^F/F mouse embryos have a severe disruption of the telencephalic apical junctionThe EMBO journalRepression of Irs2 by let-7 miRNAs is essential for homeostasis of the telencephalic neuroepithelium.PMC7604626PGC2018-102172-B-I00SEV-2017-0723SAF2015-69168-R309633Tomasello UDori MNomura YCalegari FPrieto-Colomina AFernandez VBorrell VMartinez-Martinez MALopez-Atalaya JPCardenas ASoler RfalseRepression of Irs2 by let-7 miRNAs is essential for homeostasis of the telencephalic neuroepithelium.Structural integrity and cellular homeostasis of the embryonic stem cell niche are critical for normal tissue development. In the telencephalic neuroepithelium, this is controlled in part by cell adhesion molecules and regulators of progenitor cell lineage, but the specific orchestration of these processes remains unknown. Here, we studied the role of microRNAs in the embryonic telencephalon as key regulators of gene expression. By using the early recombiner Rx-Cre mouse, we identify novel and critical roles of miRNAs in early brain development, demonstrating they are essential to preserve the cellular homeostasis and structural integrity of the telencephalic neuroepithelium. We show that Rx-Cre;Dicer^F/F mouse embryos have a severe disruption of the telencephalic apical junction2020-01-01T00:00:00Z2020 Nov2026-05-27T17:23:46.59Z2020-11-08T09:42:46ZS-EPMC76046263298570510.15252/embj.2020105479