biostudies-literatureOpuni KFMDeutscher Akademischer AustauschdienstEuropean Union14987-14997https://www.ebi.ac.uk/biostudies/studies/S-EPMC7606685biostudies-literatureUnknown295(44)Rapid diagnostic tests are first-line assays for diagnosing infectious diseases, such as malaria. To minimize false positive and false negative test results in population-screening assays, high-quality reagents and well-characterized antigens and antibodies are needed. An important property of antigen-antibody binding is recognition specificity, which best can be estimated by mapping an antibody's epitope on the respective antigen. We have cloned a malarial antigen-containing fusion protein, MBP-<i>pf</i>MSP1₁₉, in <i>Escherichia coli</i>, which then was structurally and functionally characterized before and after high pressure-assisted enzymatic digestion. We then used our previously developed method, intact transition epitope mapping-targeted high-energy rupture of extracted eThe Journal of biological chemistryITEM-THREE analysis of a monoclonal anti-malaria antibody reveals its assembled epitope on the <i>pf</i>MSP1₁₉ antigen.PMC7606685EFRE-UHROM 99156606491548123Thiesen HJAlef AGlocker MORuss MOpuni KFMWeresow MDanquah BDReepmeyer MLorenz PKoy CfalseITEM-THREE analysis of a monoclonal anti-malaria antibody reveals its assembled epitope on the <i>pf</i>MSP1₁₉ antigen.Rapid diagnostic tests are first-line assays for diagnosing infectious diseases, such as malaria. To minimize false positive and false negative test results in population-screening assays, high-quality reagents and well-characterized antigens and antibodies are needed. An important property of antigen-antibody binding is recognition specificity, which best can be estimated by mapping an antibody's epitope on the respective antigen. We have cloned a malarial antigen-containing fusion protein, MBP-<i>pf</i>MSP1₁₉, in <i>Escherichia coli</i>, which then was structurally and functionally characterized before and after high pressure-assisted enzymatic digestion. We then used our previously developed method, intact transition epitope mapping-targeted high-energy rupture of extracted e2020-01-01T00:00:00Z2020 Oct2026-05-09T01:45:28.343Z2022-02-11T12:23:51.071ZS-EPMC76066853284802010.1074/jbc.ra120.01480210.1074/jbc.RA120.014802