{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Willcocks SJ"],"funding":["Wellcome Trust","the Wellcome Trust, UK"],"pagination":["241-257"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7611010"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15"],"pubmed_abstract":["<b>Aim:</b> We sought to characterize the contribution of the <i>O</i>-OTase, PglL, to virulence in two <i>Burkholderia</i> spp. by comparing isogenic mutants in <i>Burkholderia pseudomallei</i> with the related species, <i>Burkholderia thailandensis</i>. <b>Materials & methods:</b> We utilized an array of <i>in vitro</i> assays in addition to <i>Galleria mellonella</i> and murine <i>in vivo</i> models to assess virulence of the mutant and wild-type strains in each <i>Burkholderia</i> species. <b>Results:</b> We found that <i>pglL</i> contributes to biofilm and twitching motility in both species. <i>PglL</i> uniquely affected morphology; cell invasion; intracellular motility; plaque formation and intergenus competition in <i>B. pseudomallei</i>. This mutant was attenuated in the murine mod"],"journal":["Future microbiology"],"pubmed_title":["Virulence of the emerging pathogen, &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt;, depends upon the &lt;i&gt;O&lt;/i&gt;-linked oligosaccharyltransferase, PglL."],"pmcid":["PMC7611010"],"funding_grant_id":["102979","102979/Z/13/Z"],"pubmed_authors":["Cia F","Cuccui J","Denman C","McCarthy E","Wren BW","Willcocks SJ"],"additional_accession":[]},"is_claimable":false,"name":"Virulence of the emerging pathogen, &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt;, depends upon the &lt;i&gt;O&lt;/i&gt;-linked oligosaccharyltransferase, PglL.","description":"<b>Aim:</b> We sought to characterize the contribution of the <i>O</i>-OTase, PglL, to virulence in two <i>Burkholderia</i> spp. by comparing isogenic mutants in <i>Burkholderia pseudomallei</i> with the related species, <i>Burkholderia thailandensis</i>. <b>Materials & methods:</b> We utilized an array of <i>in vitro</i> assays in addition to <i>Galleria mellonella</i> and murine <i>in vivo</i> models to assess virulence of the mutant and wild-type strains in each <i>Burkholderia</i> species. <b>Results:</b> We found that <i>pglL</i> contributes to biofilm and twitching motility in both species. <i>PglL</i> uniquely affected morphology; cell invasion; intracellular motility; plaque formation and intergenus competition in <i>B. pseudomallei</i>. This mutant was attenuated in the murine mod","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2026-05-08T00:20:00.015Z","creation":"2025-05-18T12:39:46.97Z"},"accession":"S-EPMC7611010","cross_references":{"pubmed":["32271107"],"doi":["10.2217/fmb-2019-0165"]}}