biostudies-literatureHellberg IEuropean Research Council482-492https://www.ebi.ac.uk/biostudies/studies/S-EPMC7614369biostudies-literatureUnknown31(4)<h4>Objective</h4>Meniscal calcifications are associated with the pathogenesis of knee osteoarthritis (OA). We propose a micro-computed tomography (μCT) based 3D analysis of meniscal calcifications ex vivo, including a new grading system.<h4>Method</h4>Human medial and lateral menisci were obtained from 10 patients having total knee replacement for medial compartment OA and 10 deceased donors without knee OA (healthy references). The samples were fixed; one subsection was imaged with μCT, and the adjacent tissue was processed for histological evaluation. Calcifications were examined from the reconstructed 3D μCT images, and a new grading system was developed. To validate the grading system, meniscal calcification volumes (CV_M) were quantitatively analyzed and compared between thOsteoarthritis and cartilage3D analysis and grading of calcifications from ex vivo human meniscus.PMC7614369336267771121Finnila MAJJonsson EKarjalainen VPTurkiewicz AHughes VHellberg ITjornstrand JOnnerfjord PSaarakkala SEnglund Mfalse3D analysis and grading of calcifications from ex vivo human meniscus.<h4>Objective</h4>Meniscal calcifications are associated with the pathogenesis of knee osteoarthritis (OA). We propose a micro-computed tomography (μCT) based 3D analysis of meniscal calcifications ex vivo, including a new grading system.<h4>Method</h4>Human medial and lateral menisci were obtained from 10 patients having total knee replacement for medial compartment OA and 10 deceased donors without knee OA (healthy references). The samples were fixed; one subsection was imaged with μCT, and the adjacent tissue was processed for histological evaluation. Calcifications were examined from the reconstructed 3D μCT images, and a new grading system was developed. To validate the grading system, meniscal calcification volumes (CV_M) were quantitatively analyzed and compared between th2023-01-01T00:00:00Z2023 Apr2025-04-21T22:32:16.089Z2025-04-05T18:50:54.476ZS-EPMC76143693635692810.1016/j.joca.2022.10.016