biostudies-literatureFeng CAustrian Science Fund FWFDeutsche Forschungsgemeinschaft616-630https://www.ebi.ac.uk/biostudies/studies/S-EPMC7614470biostudies-literatureUnknown9(4)During meiotic prophase I, sister chromatids are arranged in a loop-base array along a proteinaceous structure, called the meiotic chromosome axis. This structure is essential for synapsis and meiotic recombination progression and hence formation of genetically diverse gametes. Proteomic studies in plants aiming to unravel the composition and regulation of meiotic axes are constrained by limited meiotic cells embedded in floral organs. Here we report TurboID (TbID)-based proximity labelling (PL) in meiotic cells of Arabidopsis thaliana. TbID fusion to the two meiotic chromosome axis proteins ASY1 and ASY3 enabled the identification of their proximate 'interactomes' based on affinity purification coupled with mass spectrometry. We identified 39 ASY1 and/or ASY3 proximate candidates coveringNature plantsTurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana.PMC7614470I 3686I 3686-B25ERA-CAPS I 3686-B25-MEIOREC international projectHE 7950/1-1 in frame of the ERA-CAPS MEIORECCuacos MSchubert VHudecz OLorenz JWang BWang RMechtler KFeng CRoitinger EHeckmann SfalseTurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana.During meiotic prophase I, sister chromatids are arranged in a loop-base array along a proteinaceous structure, called the meiotic chromosome axis. This structure is essential for synapsis and meiotic recombination progression and hence formation of genetically diverse gametes. Proteomic studies in plants aiming to unravel the composition and regulation of meiotic axes are constrained by limited meiotic cells embedded in floral organs. Here we report TurboID (TbID)-based proximity labelling (PL) in meiotic cells of Arabidopsis thaliana. TbID fusion to the two meiotic chromosome axis proteins ASY1 and ASY3 enabled the identification of their proximate 'interactomes' based on affinity purification coupled with mass spectrometry. We identified 39 ASY1 and/or ASY3 proximate candidates covering2023-01-01T00:00:00Z2023 Apr2025-04-04T02:25:16.528Z2025-02-19T03:43:54.276ZS-EPMC76144703691489810.1038/s41477-023-01371-7