{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stempels FC"],"funding":["European Research Council","ZonMw"],"pagination":["113228"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7614694"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["502"],"pubmed_abstract":["Quantitative detection of T cell proliferation is an important readout in immunology research, as it is one of the hallmarks of T cell activation. Fluorescence-based methods for T cell proliferation mostly rely on the usage of probes that non-specifically conjugate to free primary amine groups in cells. Each cell division then results in a two-fold dilution of the probes which is detectable with flow cytometry. However, questions have been raised about cytotoxicity of these dilution-based T cell proliferation probes and they potentially affect T cell activation. An alternative assay relies on the incorporation of the uridine analog BrdU in the DNA of dividing T cells that can be detected with an antibody, but this requires harsh fixation and denaturation conditions. Recently, a new assay f"],"journal":["Journal of immunological methods"],"pubmed_title":["A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue."],"pmcid":["PMC7614694"],"funding_grant_id":["09120011910001","862137","04510011910005"],"pubmed_authors":["Bianchi F","de Wit AS","Baranov MV","van den Bogaart G","Swierstra MS","Maassen S","Stempels FC"],"additional_accession":[]},"is_claimable":false,"name":"A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue.","description":"Quantitative detection of T cell proliferation is an important readout in immunology research, as it is one of the hallmarks of T cell activation. Fluorescence-based methods for T cell proliferation mostly rely on the usage of probes that non-specifically conjugate to free primary amine groups in cells. Each cell division then results in a two-fold dilution of the probes which is detectable with flow cytometry. However, questions have been raised about cytotoxicity of these dilution-based T cell proliferation probes and they potentially affect T cell activation. An alternative assay relies on the incorporation of the uridine analog BrdU in the DNA of dividing T cells that can be detected with an antibody, but this requires harsh fixation and denaturation conditions. Recently, a new assay f","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-04T02:25:39.123Z","creation":"2025-02-19T04:29:28.13Z"},"accession":"S-EPMC7614694","cross_references":{"pubmed":["35074315"],"doi":["10.1016/j.jim.2022.113228"]}}