{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(3)"],"submitter":["Hou Y"],"pubmed_abstract":["Ca<sup>2+</sup> sparks constitute the fundamental units of Ca<sup>2+</sup> release in cardiomyocytes. Here we investigate how ryanodine receptors (RyRs) collectively generate these events by employing a transgenic mouse with a photo-activated label on RyR2. This allowed correlative imaging of RyR localization, by super-resolution Photo-Activated Localization Microscopy, and Ca<sup>2+</sup> sparks, by high-speed imaging. Two populations of Ca<sup>2+</sup> sparks were observed: stationary events and \"travelling\" events that spread between neighbouring RyR clusters. Travelling sparks exhibited up to 8 distinct releases, sourced from local or distal junctional sarcoplasmic reticulum. Quantitative analyses showed that sparks may be triggered by any number of RyRs within a cluster, and that acute β-adrenergic stimulation augments intra-cluster RyR recruitment to generate larger events. In contrast, RyR \"dispersion\" during heart failure facilitates the generation of travelling sparks. Thus, RyRs cooperatively generate Ca<sup>2+</sup> sparks in a complex, malleable fashion, and channel organization regulates the propensity for local propagation of Ca<sup>2+</sup> release."],"journal":["Nature cardiovascular research"],"pagination":["251-267"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7616007"],"repository":["biostudies-literature"],"pubmed_title":["Live-cell photo-activated localization microscopy correlates nanoscale ryanodine receptor configuration to calcium sparks in cardiomyocytes."],"pmcid":["PMC7616007"],"pubmed_authors":["Louch WE","Li J","Manfra O","Sjaastad I","Le C","Laasmaa M","Jones PP","Norden ES","Zhang L","Hou Y","Shen X","Soeller C"],"additional_accession":[]},"is_claimable":false,"name":"Live-cell photo-activated localization microscopy correlates nanoscale ryanodine receptor configuration to calcium sparks in cardiomyocytes.","description":"Ca<sup>2+</sup> sparks constitute the fundamental units of Ca<sup>2+</sup> release in cardiomyocytes. Here we investigate how ryanodine receptors (RyRs) collectively generate these events by employing a transgenic mouse with a photo-activated label on RyR2. This allowed correlative imaging of RyR localization, by super-resolution Photo-Activated Localization Microscopy, and Ca<sup>2+</sup> sparks, by high-speed imaging. Two populations of Ca<sup>2+</sup> sparks were observed: stationary events and \"travelling\" events that spread between neighbouring RyR clusters. Travelling sparks exhibited up to 8 distinct releases, sourced from local or distal junctional sarcoplasmic reticulum. Quantitative analyses showed that sparks may be triggered by any number of RyRs within a cluster, and that acute β-adrenergic stimulation augments intra-cluster RyR recruitment to generate larger events. In contrast, RyR \"dispersion\" during heart failure facilitates the generation of travelling sparks. Thus, RyRs cooperatively generate Ca<sup>2+</sup> sparks in a complex, malleable fashion, and channel organization regulates the propensity for local propagation of Ca<sup>2+</sup> release.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-06-02T08:33:06.959Z","creation":"2025-04-07T02:07:27.864Z"},"accession":"S-EPMC7616007","cross_references":{"pubmed":["38803363"],"doi":["10.1038/s44161-022-00199-2"]}}