{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kar R"],"funding":["Department of Biotechnology-Junior Research Fellowship","DBT/Wellcome Trust India Alliance Fellowship","DBT-NII","DBT/Wellcome Trust India Alliance","Wellcome Trust"],"pagination":["420-439"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7616077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["172(3)"],"pubmed_abstract":["Latent human cytomegalovirus (hCMV) infection can pose a serious threat of reactivation and disease occurrence in immune-compromised individuals. Although T cells are at the core of the protective immune response to hCMV infection, a detailed characterization of different T cell subsets involved in hCMV immunity is lacking. Here, in an unbiased manner, we characterized over 8000 hCMV-reactive peripheral memory T cells isolated from seropositive human donors, at a single-cell resolution by analysing their single-cell transcriptomes paired with the T cell antigen receptor (TCR) repertoires. The hCMV-reactive T cells were highly heterogeneous and consisted of different developmental and functional memory T cell subsets such as, long-term memory precursors and effectors, T helper-17, T regulatory cells (T<sub>REGs</sub>) and cytotoxic T lymphocytes (CTLs) of both CD4 and CD8 origin. The hCMV-specific T<sub>REGs</sub>, in addition to being enriched for molecules known for their suppressive functions, showed enrichment for the interferon response signature gene sets. The hCMV-specific CTLs were of two types, the pre-effector- and effector-like. The co-clustering of hCMV-specific CD4-CTLs and CD8-CTLs in both pre-effector as well as effector clusters suggest shared transcriptomic signatures between them. The huge TCR clonal expansion of cytotoxic clusters suggests a dominant role in the protective immune response to CMV. The study uncovers the heterogeneity in the hCMV-specific memory T cells revealing many functional subsets with potential implications in better understanding of hCMV-specific T cell immunity. The data presented can serve as a knowledge base for designing vaccines and therapeutics."],"journal":["Immunology"],"pubmed_title":["Single-cell transcriptomic and T cell antigen receptor analysis of human cytomegalovirus (hCMV)-specific memory T cells reveals effectors and pre-effectors of CD8&lt;sup&gt;+&lt;/sup&gt;- and CD4&lt;sup&gt;+&lt;/sup&gt;-cytotoxic T cells."],"pmcid":["PMC7616077"],"funding_grant_id":["DBT/JRF/BET-19/I/2019/AL/89","IA/I/18/2/504012"],"pubmed_authors":["Kar R","Sharma A","Patil VS","Sinha S","Arimbasseri GA","Chattopadhyay S","Sharma K"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomic and T cell antigen receptor analysis of human cytomegalovirus (hCMV)-specific memory T cells reveals effectors and pre-effectors of CD8&lt;sup&gt;+&lt;/sup&gt;- and CD4&lt;sup&gt;+&lt;/sup&gt;-cytotoxic T cells.","description":"Latent human cytomegalovirus (hCMV) infection can pose a serious threat of reactivation and disease occurrence in immune-compromised individuals. Although T cells are at the core of the protective immune response to hCMV infection, a detailed characterization of different T cell subsets involved in hCMV immunity is lacking. Here, in an unbiased manner, we characterized over 8000 hCMV-reactive peripheral memory T cells isolated from seropositive human donors, at a single-cell resolution by analysing their single-cell transcriptomes paired with the T cell antigen receptor (TCR) repertoires. The hCMV-reactive T cells were highly heterogeneous and consisted of different developmental and functional memory T cell subsets such as, long-term memory precursors and effectors, T helper-17, T regulatory cells (T<sub>REGs</sub>) and cytotoxic T lymphocytes (CTLs) of both CD4 and CD8 origin. The hCMV-specific T<sub>REGs</sub>, in addition to being enriched for molecules known for their suppressive functions, showed enrichment for the interferon response signature gene sets. The hCMV-specific CTLs were of two types, the pre-effector- and effector-like. The co-clustering of hCMV-specific CD4-CTLs and CD8-CTLs in both pre-effector as well as effector clusters suggest shared transcriptomic signatures between them. The huge TCR clonal expansion of cytotoxic clusters suggests a dominant role in the protective immune response to CMV. The study uncovers the heterogeneity in the hCMV-specific memory T cells revealing many functional subsets with potential implications in better understanding of hCMV-specific T cell immunity. The data presented can serve as a knowledge base for designing vaccines and therapeutics.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-05-07T14:11:48.196Z","creation":"2026-04-07T22:51:21.678Z"},"accession":"S-EPMC7616077","cross_references":{"pubmed":["38501302"],"doi":["10.1111/imm.13783"]}}