{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Siriwardena D"],"funding":["Wellcome Trust","University of Cambridge Centre for Trophoblast Research"],"pagination":["1427-1446.e8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7616712"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(10)"],"pubmed_abstract":["Early human trophoblast development has remained elusive due to the inaccessibility of the early conceptus. Non-human primate models recapitulate many features of human development and allow access to early postimplantation stages. Here, we tracked the pre- to postimplantation transition of the trophoblast lineage in superficially implanting marmoset embryos in vivo. We differentiated marmoset naive pluripotent stem cells into trophoblast stem cells (TSCs), which exhibited trophoblast-specific transcriptome, methylome, differentiation potential, and long-term self-renewal. Notably, human TSC culture conditions failed to support marmoset TSC derivation, instead inducing an extraembryonic mesoderm-like fate in marmoset cells. We show that combined MEK, TGF-β/NODAL, and histone deacetylase in"],"journal":["Cell stem cell"],"pubmed_title":["Marmoset and human trophoblast stem cells differ in signaling requirements and recapitulate divergent modes of trophoblast invasion."],"pmcid":["PMC7616712"],"funding_grant_id":["203151/A/16/Z","203151/Z/16/Z","203151","WT108438/C/15/Z","206684","108438"],"pubmed_authors":["Reik W","Kohler TN","Rawlings TM","Slatery E","Ellermann AL","Linneberg-Agerholm M","Clark SJ","Zernicka-Goetz M","Siriwardena D","Weberling A","Munger C","Hollfelder F","Boroviak TE","Sasaki E","Penfold C","Bergmann S","Brickman JM","Brosens JJ","Behr R"],"additional_accession":[]},"is_claimable":false,"name":"Marmoset and human trophoblast stem cells differ in signaling requirements and recapitulate divergent modes of trophoblast invasion.","description":"Early human trophoblast development has remained elusive due to the inaccessibility of the early conceptus. Non-human primate models recapitulate many features of human development and allow access to early postimplantation stages. Here, we tracked the pre- to postimplantation transition of the trophoblast lineage in superficially implanting marmoset embryos in vivo. We differentiated marmoset naive pluripotent stem cells into trophoblast stem cells (TSCs), which exhibited trophoblast-specific transcriptome, methylome, differentiation potential, and long-term self-renewal. Notably, human TSC culture conditions failed to support marmoset TSC derivation, instead inducing an extraembryonic mesoderm-like fate in marmoset cells. We show that combined MEK, TGF-β/NODAL, and histone deacetylase in","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T02:21:13.208Z","creation":"2025-04-04T02:21:13.208Z"},"accession":"S-EPMC7616712","cross_references":{"pubmed":["39321797"],"doi":["10.1016/j.stem.2024.09.004"]}}