{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ianeselli A"],"funding":["Simons Foundation","European Research Council"],"pagination":["13155-13160"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7616952"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(37)"],"pubmed_abstract":["To understand the emergence of life, a better understanding of the physical chemistry of primordial non-equilibrium conditions is essential. Significant salt concentrations are required for the catalytic function of RNA. The separation of oligonucleotides into single strands is a difficult problem as the hydrolysis of RNA becomes a limiting factor at high temperatures. Salt concentrations modulate the melting of DNA or RNA, and its periodic modulation would enable melting and annealing cycles at low temperatures. In our experiments, a moderate temperature difference created a miniaturized water cycle, resulting in fluctuations in salt concentration, leading to melting of oligonucleotides at temperatures 20 °C below the melting temperature. This would enable the reshuffling of duplex oligonucleotides, necessary for ligation chain replication. The findings suggest an autonomous route to overcome the strand-separation problem of non-enzymatic replication in early evolution."],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Periodic Melting of Oligonucleotides by Oscillating Salt Concentrations Triggered by Microscale Water Cycles Inside Heated Rock Pores."],"pmcid":["PMC7616952"],"funding_grant_id":["787356","327125"],"pubmed_authors":["Mast CB","Ianeselli A","Braun D"],"additional_accession":[]},"is_claimable":false,"name":"Periodic Melting of Oligonucleotides by Oscillating Salt Concentrations Triggered by Microscale Water Cycles Inside Heated Rock Pores.","description":"To understand the emergence of life, a better understanding of the physical chemistry of primordial non-equilibrium conditions is essential. Significant salt concentrations are required for the catalytic function of RNA. The separation of oligonucleotides into single strands is a difficult problem as the hydrolysis of RNA becomes a limiting factor at high temperatures. Salt concentrations modulate the melting of DNA or RNA, and its periodic modulation would enable melting and annealing cycles at low temperatures. In our experiments, a moderate temperature difference created a miniaturized water cycle, resulting in fluctuations in salt concentration, leading to melting of oligonucleotides at temperatures 20 °C below the melting temperature. This would enable the reshuffling of duplex oligonucleotides, necessary for ligation chain replication. The findings suggest an autonomous route to overcome the strand-separation problem of non-enzymatic replication in early evolution.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Sep","modification":"2025-04-26T01:33:55.897Z","creation":"2025-04-06T10:14:27.682Z"},"accession":"S-EPMC7616952","cross_references":{"pubmed":["31322800"],"doi":["10.1002/anie.201907909"]}}