{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Moreno-Layseca P"],"funding":["European Research Council","DBT/Wellcome Trust India Alliance","Cancer Foundation Finland sr"],"pagination":["1073-1084"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617174"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(10)"],"pubmed_abstract":["Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route."],"journal":["Nature cell biology"],"pubmed_title":["Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis."],"pmcid":["PMC7617174"],"funding_grant_id":["IA/M/15/1/502018","200084","615258"],"pubmed_authors":["Disanza A","Moreno-Layseca P","Godbole R","Jacquemet G","Selbach M","Aepfelbacher M","Zauber H","Sommer C","Al-Akhrass H","Veltel S","Cervero P","Rae J","Kronqvist P","Jantti NZ","Conway JRW","Kallionpaa RE","Parton RG","Mayor S","Oliveira-Ferrer L","Linder S","Ivaska J","Scita G"],"additional_accession":[]},"is_claimable":false,"name":"Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis.","description":"Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-04-22T03:15:23.99Z","creation":"2025-04-03T23:18:44.785Z"},"accession":"S-EPMC7617174","cross_references":{"pubmed":["34616024"],"doi":["10.1038/s41556-021-00767-x"]}}