biostudies-literatureClare CUKRI | Biotechnology and Biological Sciences Research CouncilUKRI | Engineering and Physical Sciences Research Council (EPSRC)European Research CouncilUKRI | Engineering and Physical Sciences Research CouncilWellcome TrustBiotechnology and Biological Sciences Research Councile0026924https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617246biostudies-literatureUnknown206(12)Bacterial microcompartments (BMCs) are self-assembled protein structures often utilized by bacteria as a modular metabolic unit, enabling the catalysis and utilization of less common carbon and nitrogen sources within a self-contained compartment. The <i>ethanolamine (EA) utilization (eut)</i> BMC has been widely demonstrated in enteropathogens, such as <i>Salmonella enterica</i>, and current research is exploring its activity in the commensal species that populate the human gut. <i>Escherichia coli</i> Nissle 1917 (EcN) is a strong colonizer and probiotic in gut microbial communities and has been used extensively for microbiome engineering. In this study, the utilization of ethanolamine as a sole carbon source and the formation of the <i>eut</i> BMC in EcN were demonstrated through growthJournal of bacteriologyBacterial microcompartment utilization in the human commensal <i>Escherichia coli</i> Nissle 1917.PMC7617246770835209409EP/R013756/1209409/Z/17/ZBB/T008709/1EP/W004674/1Rutter JWFrank SBarnes CPClare CFedorec AJHfalseBacterial microcompartment utilization in the human commensal <i>Escherichia coli</i> Nissle 1917.Bacterial microcompartments (BMCs) are self-assembled protein structures often utilized by bacteria as a modular metabolic unit, enabling the catalysis and utilization of less common carbon and nitrogen sources within a self-contained compartment. The <i>ethanolamine (EA) utilization (eut)</i> BMC has been widely demonstrated in enteropathogens, such as <i>Salmonella enterica</i>, and current research is exploring its activity in the commensal species that populate the human gut. <i>Escherichia coli</i> Nissle 1917 (EcN) is a strong colonizer and probiotic in gut microbial communities and has been used extensively for microbiome engineering. In this study, the utilization of ethanolamine as a sole carbon source and the formation of the <i>eut</i> BMC in EcN were demonstrated through growth2024-01-01T00:00:00Z2024 Dec2026-07-15T21:56:00.655Z2025-04-04T01:36:26.603ZS-EPMC76172463963625410.1128/jb.00269-24