biostudies-literatureJovanovic ZMedical Research CouncilEuropean Commission FP7Wellcome Trust915-25https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617485biostudies-literatureUnknown22(8)Leptin plays a major role in coordinating the integrated response of the brain to changes in nutritional state. Leptin receptor expressing neurones within the arcuate nucleus (ARC) of the hypothalamus sense circulating leptin and densely innervate other regions of the hypothalamus, including the paraventricular nucleus (PVN). In the ARC, leptin is known to alter the expression of genes with important roles in the control of energy balance, and the aim of the present study was to obtain a more comprehensive picture of the action of leptin in these nuclei. Mice were ad libitum fed, or fasted for 48 h when receiving either sham or i.p. leptin treatment. We used laser capture microdissection and microarrays to identify leptin-regulated transcripts within the ARC. Expression of 639 genes are inJournal of neuroendocrinologyIdentification of the global transcriptomic response of the hypothalamic arcuate nucleus to fasting and leptin.PMC7617485512013FP7_241592G0600717Jovanovic ZLam BYYeo GSO'Rahilly STung YCfalseIdentification of the global transcriptomic response of the hypothalamic arcuate nucleus to fasting and leptin.Leptin plays a major role in coordinating the integrated response of the brain to changes in nutritional state. Leptin receptor expressing neurones within the arcuate nucleus (ARC) of the hypothalamus sense circulating leptin and densely innervate other regions of the hypothalamus, including the paraventricular nucleus (PVN). In the ARC, leptin is known to alter the expression of genes with important roles in the control of energy balance, and the aim of the present study was to obtain a more comprehensive picture of the action of leptin in these nuclei. Mice were ad libitum fed, or fasted for 48 h when receiving either sham or i.p. leptin treatment. We used laser capture microdissection and microarrays to identify leptin-regulated transcripts within the ARC. Expression of 639 genes are in2010-01-01T00:00:00Z2010 Aug2026-06-03T04:43:32.121Z2025-04-04T14:47:32.222ZS-EPMC76174852055337010.1111/j.1365-2826.2010.02026.x