{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma M"],"funding":["Medical Research Council","European Commission FP7","Wellcome Trust"],"pagination":["183-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617487"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["444(2)"],"pubmed_abstract":["Genomewide-association studies have revealed that SNPs (single nucleotide polymorphisms) in FTO (fat mass and obesity-associated) are robustly associated with BMI (body mass index) and obesity. FTO is an Fe(II) 2-OG (2-oxoglutarate)-dependent dioxygenase that can demethylate 3-meT (3-methylthymine) in single-stranded DNA, as well as 3-meU (3-methyluracil) and N6-methyl adenosine in RNA. In the present paper we describe the development of an RNase-cleavage assay measuring the demethylation activity of FTO on 3-meU. RNase A cleaves at the 3'-end of pyrimidines, including uracil, and a methyl group at position three of uracil inhibits cleavage. An oligonucleotide probe was designed consisting of a DNA stem, an RNA loop containing a single 3-meU as the only RNase A-cleavage site, a fluorescent"],"journal":["The Biochemical journal"],"pubmed_title":["Kinetic analysis of FTO (fat mass and obesity-associated) reveals that it is unlikely to function as a sensor for 2-oxoglutarate."],"pmcid":["PMC7617487"],"funding_grant_id":["FP7_266408","G0600717B","G0900554","241592","084812","FP7_241592","G0600717"],"pubmed_authors":["Ron D","Harding HP","Ma M","Yeo GS","O'Rahilly S"],"additional_accession":[]},"is_claimable":false,"name":"Kinetic analysis of FTO (fat mass and obesity-associated) reveals that it is unlikely to function as a sensor for 2-oxoglutarate.","description":"Genomewide-association studies have revealed that SNPs (single nucleotide polymorphisms) in FTO (fat mass and obesity-associated) are robustly associated with BMI (body mass index) and obesity. FTO is an Fe(II) 2-OG (2-oxoglutarate)-dependent dioxygenase that can demethylate 3-meT (3-methylthymine) in single-stranded DNA, as well as 3-meU (3-methyluracil) and N6-methyl adenosine in RNA. In the present paper we describe the development of an RNase-cleavage assay measuring the demethylation activity of FTO on 3-meU. RNase A cleaves at the 3'-end of pyrimidines, including uracil, and a methyl group at position three of uracil inhibits cleavage. An oligonucleotide probe was designed consisting of a DNA stem, an RNA loop containing a single 3-meU as the only RNase A-cleavage site, a fluorescent","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jun","modification":"2025-04-18T14:00:54.49Z","creation":"2025-04-04T14:46:59.587Z"},"accession":"S-EPMC7617487","cross_references":{"pubmed":["22435707"],"doi":["10.1042/bj20120065","10.1042/BJ20120065"]}}