biostudies-literatureLiu YDBT/Wellcome Trust India Alliance12293-12300https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617626biostudies-literatureUnknown15(50)Fluorescence probes play crucial roles in unraveling the structure and dynamics of cell membranes including membrane fluidity, polarity, and lipid molecule ordering. The fluorescence lifetime of probes describes the average duration of time that a fluorescent molecule remains in an excited state before returning to the ground state, which is sensitive to environmental changes. However, the molecular mechanism and inherent properties to determine the fluorescence lifetimes remain unexplored and inadequately studied. Furthermore, the effects of the probe on the membrane are also unclear. In this study, we investigated the interactions between probes and lipids, as well as the structural properties of probes within the outer and inner membrane of <i>Mycobacterium smegmatis</i> (<i>Msm</i>) byThe journal of physical chemistry lettersThe Molecular Mechanism of Fluorescence Lifetime of Fluorescent Probes in Cell Membranes.PMC7617626IA/I/21/1/505624Liu YDuan MMathew LYu CShu ZKapoor SFu LfalseThe Molecular Mechanism of Fluorescence Lifetime of Fluorescent Probes in Cell Membranes.Fluorescence probes play crucial roles in unraveling the structure and dynamics of cell membranes including membrane fluidity, polarity, and lipid molecule ordering. The fluorescence lifetime of probes describes the average duration of time that a fluorescent molecule remains in an excited state before returning to the ground state, which is sensitive to environmental changes. However, the molecular mechanism and inherent properties to determine the fluorescence lifetimes remain unexplored and inadequately studied. Furthermore, the effects of the probe on the membrane are also unclear. In this study, we investigated the interactions between probes and lipids, as well as the structural properties of probes within the outer and inner membrane of <i>Mycobacterium smegmatis</i> (<i>Msm</i>) by2024-01-01T00:00:00Z2024 Dec2025-07-04T03:05:42.52Z2025-07-04T03:05:42.52ZS-EPMC76176263963970510.1021/acs.jpclett.4c02731