{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Dhanya SK"],"funding":["DST-SERB Start Iup research grant","DBT-MK Bhan Young Researcher Fellowship","DBT-inStem Core funds","DBT/Wellcome Trust India Alliance","Wellcome Trust","DBT Har Gobind Khorana Innovative Young Biologist Fellowship","DBT/Wellcome Trust India Alliance:Intermediate Career Fellowship","Pratiksha Grant to inStem"],"pubmed_abstract":["Chromatin regulation plays a crucial role in neocortical neurogenesis, and mutations in chromatin modifiers are linked to neurodevelopmental disorders. RBBP4 is a core subunit of several chromatin-modifying complexes; however, its functional role and genome-wide occupancy profile in the neocortical primordium are unknown. To address this, we performed RBBP4 knockdown using CRISPR/Cas9 on neocortical progenitors derived from mice of both sexes at embryonic age 12.5 during deep-layer neurogenesis. Our study demonstrates that downregulation of RBBP4 in the E12.5 neocortical progenitors reduced neuronal output, specifically affecting CTIP2-expressing neurons. We demonstrate that RBBP4 plays an essential role in regulating neocortical progenitor proliferation. However, overexpression of RBBP4 a"],"journal":["eNeuro"],"pagination":["ENEURO.0391-23.2024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617683"],"repository":["biostudies-literature"],"pubmed_title":["Histone-binding protein RBBP4 is necessary to promote neurogenesis in the developing mouse neocortical progenitors."],"pmcid":["PMC7617683"],"funding_grant_id":["BT/13/IYBA/2020/02","SRG/2020/001612","IA/I/19/1/504288","HRD-12/4/2020-AFS-DBT-13815"],"pubmed_authors":["Dhanya SK","Mohanty S","Swathi KS","Kalia K","Azam T","Muralidharan B","D'Souza L","Channakkar AS","Reddy PC"],"additional_accession":[]},"is_claimable":false,"name":"Histone-binding protein RBBP4 is necessary to promote neurogenesis in the developing mouse neocortical progenitors.","description":"Chromatin regulation plays a crucial role in neocortical neurogenesis, and mutations in chromatin modifiers are linked to neurodevelopmental disorders. RBBP4 is a core subunit of several chromatin-modifying complexes; however, its functional role and genome-wide occupancy profile in the neocortical primordium are unknown. To address this, we performed RBBP4 knockdown using CRISPR/Cas9 on neocortical progenitors derived from mice of both sexes at embryonic age 12.5 during deep-layer neurogenesis. Our study demonstrates that downregulation of RBBP4 in the E12.5 neocortical progenitors reduced neuronal output, specifically affecting CTIP2-expressing neurons. We demonstrate that RBBP4 plays an essential role in regulating neocortical progenitor proliferation. However, overexpression of RBBP4 a","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-18T03:21:02.822Z","creation":"2026-04-18T03:11:37.303Z"},"accession":"S-EPMC7617683","cross_references":{"pubmed":["39592227"],"doi":["10.1523/ENEURO.0391-23.2024"]}}