{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jabeen MF"],"funding":["NIHR Oxford Biomedical Research Centre","Medical Research Council","Wellcome Trust","National Institute for Health Research Southampton Biomedical Research Centre","NIHR Primary Care Research Network"],"pagination":["2966-2980"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7617782"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["79(11)"],"pubmed_abstract":["<h4>Background</h4>The airway microbiome in severe asthma has not been characterised at species-level by metagenomic sequencing, nor have the relationships between specific species and mucosal immune responses in 'type-2 low', neutrophilic asthma been defined. We performed an integrated species-level metagenomic data with inflammatory mediators to characterise prevalence of dominant potentially pathogenic organisms and host immune responses.<h4>Methods</h4>Sputum and nasal lavage samples were analysed using long-read metagenomic sequencing with Nanopore and qPCR in two cross-sectional adult severe asthma cohorts, Wessex (n = 66) and Oxford (n = 30). We integrated species-level data with clinical parameters and 39 selected airway proteins measured by immunoassay and O-link.<h4>Results</h4>T"],"journal":["Allergy"],"pubmed_title":["Species-level, metagenomic and proteomic analysis of microbe-immune interactions in severe asthma."],"pmcid":["PMC7617782"],"funding_grant_id":["211050/Z/18/z","211050"],"pubmed_authors":["Donachie G","Sanderson ND","Marchi E","Street TL","Tine M","Azim A","Pavord ID","Jabeen MF","Chauhan A","Lau LCK","Howarth PH","Barber C","Hinks TSC","Klenerman P","Brown T"],"additional_accession":[]},"is_claimable":false,"name":"Species-level, metagenomic and proteomic analysis of microbe-immune interactions in severe asthma.","description":"<h4>Background</h4>The airway microbiome in severe asthma has not been characterised at species-level by metagenomic sequencing, nor have the relationships between specific species and mucosal immune responses in 'type-2 low', neutrophilic asthma been defined. We performed an integrated species-level metagenomic data with inflammatory mediators to characterise prevalence of dominant potentially pathogenic organisms and host immune responses.<h4>Methods</h4>Sputum and nasal lavage samples were analysed using long-read metagenomic sequencing with Nanopore and qPCR in two cross-sectional adult severe asthma cohorts, Wessex (n = 66) and Oxford (n = 30). We integrated species-level data with clinical parameters and 39 selected airway proteins measured by immunoassay and O-link.<h4>Results</h4>T","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2025-07-25T03:08:19.371Z","creation":"2025-07-25T03:08:19.371Z"},"accession":"S-EPMC7617782","cross_references":{"pubmed":["39127908"],"doi":["10.1111/all.16269"]}}