biostudies-literatureUnknownMermet MMedical Research CouncilSociété Française de Rhumatologie (SFR)Wellcome TrustGlucagon-like peptide-1 (GLP-1) has previously been shown to be indispensable for optimal bone strength by acting at the bone material level. However, it was not fully clear whether the effects of GLP-1 were mediated by direct or indirect actions on bone cells. In the present study, we were unable to demonstrate the expression of the GLP-1 receptor (GLP-1r) in bone tissue at the gene expression level using qPCR and in situ hybridization, or at the protein level. Furthermore, the peripheral administration of exendin-4, a specific GLP-1r agonist, in ovariectomized (OVX) BALB/c mice enhanced post-yield displacement (18%) and energy-to-fracture (24%), as well as bone volume/total volume (BV/TV) (11%), trabecular number (Tb.N) (6%), and collagen maturity (18%). These bone effects were still obsAmerican journal of physiology. Endocrinology and metabolismhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC7618104biostudies-literatureThe GLP-1 analogue, exendin-4, improves bone material properties and strength through a central relay in ovariectomized mice.PMC7618104MC_UU_12012/3220271220271/Z/20/ZMRC_MC_UU_12012/32020-2650Mabilleau GCruciani-Guglielmacci CBiggs EDenom JMermet MWery MMieczkowska AReimann FGribble FMagnan CfalseThe GLP-1 analogue, exendin-4, improves bone material properties and strength through a central relay in ovariectomized mice.Glucagon-like peptide-1 (GLP-1) has previously been shown to be indispensable for optimal bone strength by acting at the bone material level. However, it was not fully clear whether the effects of GLP-1 were mediated by direct or indirect actions on bone cells. In the present study, we were unable to demonstrate the expression of the GLP-1 receptor (GLP-1r) in bone tissue at the gene expression level using qPCR and in situ hybridization, or at the protein level. Furthermore, the peripheral administration of exendin-4, a specific GLP-1r agonist, in ovariectomized (OVX) BALB/c mice enhanced post-yield displacement (18%) and energy-to-fracture (24%), as well as bone volume/total volume (BV/TV) (11%), trabecular number (Tb.N) (6%), and collagen maturity (18%). These bone effects were still obs2025-01-01T00:00:00Z2025 Aug2026-06-03T07:44:15.068Z2026-04-26T03:09:46.942ZS-EPMC76181044078966110.1152/ajpendo.00086.2025