biostudies-literatureCorato EEuropean Research Councilphysrevapplied.23.024031https://www.ebi.ac.uk/biostudies/studies/S-EPMC7618166biostudies-literatureUnknown23(2)High-acoustic-energy-density acoustofluidic devices are necessary to make this technology a viable option for clinical applications in the biomedical field. We present a mechanical interface that enables delivery of a high-amplitude acoustic field inside a fluid cavity by translating the vibrations from two large piezoelectric elements into a microfluidic chip. The study comprises both experimental characterization of a double-parabolic metallic acoustic waveguide and simulations of its working mechanism in two dimensions. We could focus 4.9-μm polystyrene particles at a flowrate of 5 ml/min, corresponding to an average retention time of 13.5 ms for particles in the actuated area. Moreover, we measured the acoustic energy density in the channel at stopped-flow condition, obtaining an average value of 1207 J<i>/</i>m³ and a maximum value of 2977 J<i>/</i>m³ with an input electrical power of 1.5 W. By comparing the simulation results with laser-Doppler vibrometer measurements, we confirmed that transverse sound waves play a significant role in the working mechanism of the double-parabolic structure, thus paving the way for further future optimization of the waveguide design.Physical review appliedHigh-energy-density acoustofluidic device using a double-parabolic ultrasonic transducer.PMC7618166852590Jakobsson OAugustsson PQiu WCorato EMorita TfalseHigh-energy-density acoustofluidic device using a double-parabolic ultrasonic transducer.High-acoustic-energy-density acoustofluidic devices are necessary to make this technology a viable option for clinical applications in the biomedical field. We present a mechanical interface that enables delivery of a high-amplitude acoustic field inside a fluid cavity by translating the vibrations from two large piezoelectric elements into a microfluidic chip. The study comprises both experimental characterization of a double-parabolic metallic acoustic waveguide and simulations of its working mechanism in two dimensions. We could focus 4.9-μm polystyrene particles at a flowrate of 5 ml/min, corresponding to an average retention time of 13.5 ms for particles in the actuated area. Moreover, we measured the acoustic energy density in the channel at stopped-flow condition, obtaining an average value of 1207 J<i>/</i>m³ and a maximum value of 2977 J<i>/</i>m³ with an input electrical power of 1.5 W. By comparing the simulation results with laser-Doppler vibrometer measurements, we confirmed that transverse sound waves play a significant role in the working mechanism of the double-parabolic structure, thus paving the way for further future optimization of the waveguide design.2025-01-01T00:00:00Z2025 Feb2026-06-03T20:34:28.523Z2026-05-01T03:11:12.081ZS-EPMC76181664100026810.1103/physrevapplied.23.024031