{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["El Sayyed H"],"funding":["Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["926-937.e4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7618293"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(5)"],"pubmed_abstract":["During transcription elongation, NusG aids RNA polymerase by inhibiting pausing, promoting anti-termination on rRNA operons, coupling transcription with translation on mRNA genes, and facilitating Rho-dependent termination. Despite extensive work, the in vivo functional allocation and spatial distribution of NusG remain unknown. Using single-molecule tracking and super-resolution imaging in live E. coli cells, we found NusG predominantly in a chromosome-associated population (binding to RNA polymerase in elongation complexes) and a slowly diffusing population complexed with the 30S ribosomal subunit; the latter provides a \"30S-guided\" path for NusG into transcription elongation. Only ∼10% of NusG is fast diffusing, with its mobility suggesting non-specific interactions with DNA for >50% of the time. Antibiotic treatments and deletion mutants revealed that chromosome-associated NusG participates mainly in rrn anti-termination within phase-separated transcriptional condensates and in transcription-translation coupling. This study illuminates the multiple roles of NusG and offers a guide on dissecting multi-functional machines via in vivo imaging."],"journal":["Molecular cell"],"pubmed_title":["Single-molecule tracking reveals the functional allocation, in vivo interactions, and spatial organization of universal transcription factor NusG."],"pmcid":["PMC7618293"],"funding_grant_id":["226662","BB/X015637/1","BB/S008896/1","BB/N018656/1","091911","107457","226662/Z/22/Z","110164/Z/15/Z","204684/Z/16/Z","224212/Z/21/Z"],"pubmed_authors":["El Sayyed H","Gottesman ME","Stracy M","Kapanidis AN","Pambos OJ"],"additional_accession":[]},"is_claimable":false,"name":"Single-molecule tracking reveals the functional allocation, in vivo interactions, and spatial organization of universal transcription factor NusG.","description":"During transcription elongation, NusG aids RNA polymerase by inhibiting pausing, promoting anti-termination on rRNA operons, coupling transcription with translation on mRNA genes, and facilitating Rho-dependent termination. Despite extensive work, the in vivo functional allocation and spatial distribution of NusG remain unknown. Using single-molecule tracking and super-resolution imaging in live E. coli cells, we found NusG predominantly in a chromosome-associated population (binding to RNA polymerase in elongation complexes) and a slowly diffusing population complexed with the 30S ribosomal subunit; the latter provides a \"30S-guided\" path for NusG into transcription elongation. Only ∼10% of NusG is fast diffusing, with its mobility suggesting non-specific interactions with DNA for >50% of the time. Antibiotic treatments and deletion mutants revealed that chromosome-associated NusG participates mainly in rrn anti-termination within phase-separated transcriptional condensates and in transcription-translation coupling. This study illuminates the multiple roles of NusG and offers a guide on dissecting multi-functional machines via in vivo imaging.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-23T03:12:10.212Z","creation":"2026-06-23T03:09:37.748Z"},"accession":"S-EPMC7618293","cross_references":{"pubmed":["38387461"],"doi":["10.1016/j.molcel.2024.01.025"]}}