biostudies-literatureVilla-Hernandez SWellcome Trust15-29https://www.ebi.ac.uk/biostudies/studies/S-EPMC7618384biostudies-literatureUnknown129Neuropathic pain is a particularly intractable type of chronic pain that can result from physical nerve damage due to surgery or entrapment. Here, we present data which suggest that a particular subclass of fibroblast and mural cells may be implicated in the sensory neuron dysfunction that is characteristic of this pain state. In a mouse model of traumatic painful neuropathy, we used cell sorting, nerve tissue clearing and RNA sequencing to study stromal cells. With cell sorting (n = 4 mouse nerves) and tissue clearing (n = 5), we show that fibroblasts and mural cells positive for the platelet-derived growth factor receptor beta (Pdgfrb) gene are increased in number for at least two months post-nerve damage. Moreover, single cell RNA sequencing data (n = 4) from our own lab and those of thBrain, behavior, and immunityA role for fibroblast and mural cell subsets in a nerve ligation model of neuropathic pain?PMC7618384224257/Z/21/Z218452/Z/19/Z224257WT098051Li YDavis HTaams LSVilla-Hernandez SWalker JVZebochin IShimizu FDenk FHore ZFedele LKanda TfalseA role for fibroblast and mural cell subsets in a nerve ligation model of neuropathic pain?Neuropathic pain is a particularly intractable type of chronic pain that can result from physical nerve damage due to surgery or entrapment. Here, we present data which suggest that a particular subclass of fibroblast and mural cells may be implicated in the sensory neuron dysfunction that is characteristic of this pain state. In a mouse model of traumatic painful neuropathy, we used cell sorting, nerve tissue clearing and RNA sequencing to study stromal cells. With cell sorting (n = 4 mouse nerves) and tissue clearing (n = 5), we show that fibroblasts and mural cells positive for the platelet-derived growth factor receptor beta (Pdgfrb) gene are increased in number for at least two months post-nerve damage. Moreover, single cell RNA sequencing data (n = 4) from our own lab and those of th2025-01-01T00:00:00Z2025 Oct2026-06-06T03:19:15.247Z2026-06-06T03:07:08.905ZS-EPMC76183844038174610.1016/j.bbi.2025.05.012