biostudies-literatureAnfang MSalk InstituteEuropean Research CouncilIsrael Science FoundationNational Science FoundationUniversity of Chinese Academy of Scienceskiaf682https://www.ebi.ac.uk/biostudies/studies/S-EPMC7618693biostudies-literatureUnknown200(2)In plants, both developmental processes and environmental responses are spatiotemporally regulated by an assembly of signaling molecules such as hormones, secondary metabolites, and ions. The ability of these signaling molecules to move within and across plant tissues is essential for various developmental cues. However, the characterization of transported signaling molecules and their translocation mechanisms is difficult due to the functional redundancy of plant genomes and shortcomings in methodologies. Here, we report our development of the Multi Targeted AmiRNA Cell type-specific Transportome-scale (mTACT) toolbox, which can be used to reveal phenotypic plasticity in plants. mTACT is based on a large set of artificial microRNAs (amiRNAs), each designed to optimally target multiple memPlant physiologymTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.PMC7618693757683101118769Ben Yaakov SSchroeder JIFichman YShani EProcko CMayrose IHaj Yahya RChory JBar HZhang YSu NBinenbaum JShafir AAnfang MYu XfalsemTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.In plants, both developmental processes and environmental responses are spatiotemporally regulated by an assembly of signaling molecules such as hormones, secondary metabolites, and ions. The ability of these signaling molecules to move within and across plant tissues is essential for various developmental cues. However, the characterization of transported signaling molecules and their translocation mechanisms is difficult due to the functional redundancy of plant genomes and shortcomings in methodologies. Here, we report our development of the Multi Targeted AmiRNA Cell type-specific Transportome-scale (mTACT) toolbox, which can be used to reveal phenotypic plasticity in plants. mTACT is based on a large set of artificial microRNAs (amiRNAs), each designed to optimally target multiple mem2026-01-01T00:00:00Z2026 Feb2026-07-16T09:40:00.639Z2026-07-09T10:47:04.145ZS-EPMC76186934147453310.1093/plphys/kiaf682