{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Useckaite Z"],"funding":["Irish Research Council","Science Foundation Ireland"],"pubmed_abstract":["MET pathway is an important actionable target across many solid tumour types and several MET inhibitors have been developed. Extracellular vesicles (EVs) are proposed to be mini-maps of their cells of origin. However, the potential of EVs to report on the MET status of their cells of origin is unknown. After applying three proposed methods of EV separation from medium conditioned by three cell lines of known MET status, this study used an extensive range of methodologies to fundamentally characterise the resulting particles (nanoparticle tracking analysis, TEM, flow cytometry, immunoblotting) and their MET status (RT-qPCR and ELISAs). The results indicated that ultracentrifugation on density-gradient (UC-DG) consistently produced the most reliable data with regards to purest EVs. EV cargo "],"journal":["Scientific reports"],"pagination":["19020"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7642384"],"repository":["biostudies-literature"],"pubmed_title":["Extracellular vesicles report on the MET status of their cells of origin regardless of the method used for their isolation."],"pmcid":["PMC7642384"],"pubmed_authors":["Mukhopadhya A","Useckaite Z","Moran B","O'Driscoll L"],"additional_accession":[]},"is_claimable":false,"name":"Extracellular vesicles report on the MET status of their cells of origin regardless of the method used for their isolation.","description":"MET pathway is an important actionable target across many solid tumour types and several MET inhibitors have been developed. Extracellular vesicles (EVs) are proposed to be mini-maps of their cells of origin. However, the potential of EVs to report on the MET status of their cells of origin is unknown. After applying three proposed methods of EV separation from medium conditioned by three cell lines of known MET status, this study used an extensive range of methodologies to fundamentally characterise the resulting particles (nanoparticle tracking analysis, TEM, flow cytometry, immunoblotting) and their MET status (RT-qPCR and ELISAs). The results indicated that ultracentrifugation on density-gradient (UC-DG) consistently produced the most reliable data with regards to purest EVs. EV cargo ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2025-04-05T15:14:15.556Z","creation":"2020-11-09T08:48:07Z"},"accession":"S-EPMC7642384","cross_references":{"pubmed":["33149187"],"doi":["10.1038/s41598-020-75817-9"]}}