{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun Z"],"funding":["National Natural Science Foundation of China"],"pagination":["6906-6919"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7644462"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(45)"],"pubmed_abstract":["RNA methylation is an important epigenetic modification. Recent studies on RNA methylation mainly focus on the m<sup>6</sup>A modification of mRNA, but very little is known about the m<sup>5</sup>C modification. NSUN2 is an RNA methyltransferase responsible for the m<sup>5</sup>C modification of multiple RNAs. In this study, we knocked down the NSUN2 gene in HepG2 cells by CRISPR/Cas9 technology and performed high-throughput RNA-BisSeq. An important tumor-related lncRNA H19 was identified to be targeted by NSUN2. Studies have shown that the expression of H19 lncRNA is abnormally elevated and has a carcinogenic effect in many types of tumors. Our results demonstrated that m<sup>5</sup>C modification of H19 lncRNA can increase its stability. Interestingly, m<sup>5</sup>C-modified H19 lncRNA "],"journal":["Oncogene"],"pubmed_title":["Aberrant NSUN2-mediated m<sup>5</sup>C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma."],"pmcid":["PMC7644462"],"funding_grant_id":["91540117","U1604281"],"pubmed_authors":["Liu Y","Guo M","Xue S","Hu X","Cui H","Zhang M","Xu H","Chen S","Sun Z"],"additional_accession":[]},"is_claimable":false,"name":"Aberrant NSUN2-mediated m<sup>5</sup>C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma.","description":"RNA methylation is an important epigenetic modification. Recent studies on RNA methylation mainly focus on the m<sup>6</sup>A modification of mRNA, but very little is known about the m<sup>5</sup>C modification. NSUN2 is an RNA methyltransferase responsible for the m<sup>5</sup>C modification of multiple RNAs. In this study, we knocked down the NSUN2 gene in HepG2 cells by CRISPR/Cas9 technology and performed high-throughput RNA-BisSeq. An important tumor-related lncRNA H19 was identified to be targeted by NSUN2. Studies have shown that the expression of H19 lncRNA is abnormally elevated and has a carcinogenic effect in many types of tumors. Our results demonstrated that m<sup>5</sup>C modification of H19 lncRNA can increase its stability. Interestingly, m<sup>5</sup>C-modified H19 lncRNA ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2025-05-18T13:30:43.438Z","creation":"2020-11-22T09:49:54Z"},"accession":"S-EPMC7644462","cross_references":{"pubmed":["32978516"],"doi":["10.1038/s41388-020-01475-w"]}}