biostudies-literatureParamel GVThe Swedish Heart and Lung FoundationÖrebro UniversityThe Knowledge Foundation19108https://www.ebi.ac.uk/biostudies/studies/S-EPMC7644683biostudies-literatureUnknown10(1)The Caspase activation and recruitment domain 8 (CARD8) protein is a component of innate immunity and overexpression of CARD8 mRNA was previously identified in atherosclerosis. However, very little is known about the regulation of CARD8 in endothelial cells and atherosclerosis. The aim of this study was to investigate CARD8 in the regulation of cytokine and chemokine expression in endothelial cells. Sections of human atherosclerotic lesions and non-atherosclerotic arteries were immunostained for CARD8 protein. Expression of CARD8 was correlated to mediators of inflammation in atherosclerotic lesions using Biobank of Karolinska Endarterectomies microarray data. The CARD8 mRNA was knocked-down in human umbilical vein endothelial cells (HUVECs) in vitro, followed by quantitative RT-PCR analysScientific reportsExpression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells.PMC7644683HÖG15Synergi18HÖG17HÖG19Ljungberg LUSirsjo AOlofsson PSFolkersen LHedin UPaulsson-Berne GParamel GVKaradimou GEremo AGFransen KfalseExpression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells.The Caspase activation and recruitment domain 8 (CARD8) protein is a component of innate immunity and overexpression of CARD8 mRNA was previously identified in atherosclerosis. However, very little is known about the regulation of CARD8 in endothelial cells and atherosclerosis. The aim of this study was to investigate CARD8 in the regulation of cytokine and chemokine expression in endothelial cells. Sections of human atherosclerotic lesions and non-atherosclerotic arteries were immunostained for CARD8 protein. Expression of CARD8 was correlated to mediators of inflammation in atherosclerotic lesions using Biobank of Karolinska Endarterectomies microarray data. The CARD8 mRNA was knocked-down in human umbilical vein endothelial cells (HUVECs) in vitro, followed by quantitative RT-PCR analys2020-01-01T00:00:00Z2020 Nov2026-05-03T07:13:07.338Z2020-11-09T08:48:27ZS-EPMC76446833315440910.1038/s41598-020-73600-4