biostudies-literatureUnknown61(13)Ru J<h4>Purpose</h4>Obstruction of the tear drainage causes a range of ocular surface disorders. Hitherto, the genetics of tear duct development and obstruction has been scarcely explored, and related animal models are lacking. This study aims to study the potential role of the Wnt/PCP pathway mediated by Prickle 1 in tear duct development and diseases.<h4>Methods</h4>A severe hypomorphic Prickle 1 mutant was generated. Histology and immunohistochemistry were performed to compare wild type, Prickle 1 hypomorphic, and null mutant tear ducts. In situ hybridization was conducted to identify the signaling components in the developing tear ducts. Three-dimensional (3D) reconstruction was used to detect the human embryonic tear duct.<h4>Results</h4>Here, we report that a severe Prickle 1 hypomorph mInvestigative ophthalmology & visual science6https://www.ebi.ac.uk/biostudies/studies/S-EPMC7645213biostudies-literatureMalformation of Tear Ducts Underlies the Epiphora and Precocious Eyelid Opening in Prickle 1 Mutant Mice: Genetic Implications for Tear Duct Genesis.PMC7645213Liu YFan JZhang JOuyang HRu JGuo DLiu CJu RWei LfalseMalformation of Tear Ducts Underlies the Epiphora and Precocious Eyelid Opening in Prickle 1 Mutant Mice: Genetic Implications for Tear Duct Genesis.<h4>Purpose</h4>Obstruction of the tear drainage causes a range of ocular surface disorders. Hitherto, the genetics of tear duct development and obstruction has been scarcely explored, and related animal models are lacking. This study aims to study the potential role of the Wnt/PCP pathway mediated by Prickle 1 in tear duct development and diseases.<h4>Methods</h4>A severe hypomorphic Prickle 1 mutant was generated. Histology and immunohistochemistry were performed to compare wild type, Prickle 1 hypomorphic, and null mutant tear ducts. In situ hybridization was conducted to identify the signaling components in the developing tear ducts. Three-dimensional (3D) reconstruction was used to detect the human embryonic tear duct.<h4>Results</h4>Here, we report that a severe Prickle 1 hypomorph m2020-01-01T00:00:00Z2020 Nov2025-04-04T10:10:52.075Z2020-11-22T09:40:21ZS-EPMC76452133314189210.1167/iovs.61.13.6