biostudies-literatureWang SNIDDK NIH HHSNational Institutes of HealthNIAMS NIH HHSdev195388https://www.ebi.ac.uk/biostudies/studies/S-EPMC7648600biostudies-literatureUnknown147(20)Between embryonic days 10.5 and 14.5, active proliferation drives rapid elongation of the murine midgut epithelial tube. Within this pseudostratified epithelium, nuclei synthesize DNA near the basal surface and move apically to divide. After mitosis, the majority of daughter cells extend a long, basally oriented filopodial protrusion, building a <i>de novo</i> path along which their nuclei can return to the basal side. WNT5A, which is secreted by surrounding mesenchymal cells, acts as a guidance cue to orchestrate this epithelial pathfinding behavior, but how this signal is received by epithelial cells is unknown. Here, we have investigated two known WNT5A receptors: ROR2 and RYK. We found that epithelial ROR2 is dispensable for midgut elongation. However, loss of <i>Ryk</i> phenocopies thDevelopment (Cambridge, England)RYK-mediated filopodial pathfinding facilitates midgut elongation.PMC7648600R01 AR055926R01 DK121166R01 AR067203R56 DK089933P30 DK034933R01 DK089933R01 DK117981Cameron LLechler TWang STsai YHUnderwood JTomlinson AJRoy JPWang EBWalton KDGumucio DLStacker SASpence JRfalseRYK-mediated filopodial pathfinding facilitates midgut elongation.Between embryonic days 10.5 and 14.5, active proliferation drives rapid elongation of the murine midgut epithelial tube. Within this pseudostratified epithelium, nuclei synthesize DNA near the basal surface and move apically to divide. After mitosis, the majority of daughter cells extend a long, basally oriented filopodial protrusion, building a <i>de novo</i> path along which their nuclei can return to the basal side. WNT5A, which is secreted by surrounding mesenchymal cells, acts as a guidance cue to orchestrate this epithelial pathfinding behavior, but how this signal is received by epithelial cells is unknown. Here, we have investigated two known WNT5A receptors: ROR2 and RYK. We found that epithelial ROR2 is dispensable for midgut elongation. However, loss of <i>Ryk</i> phenocopies th2020-01-01T00:00:00Z2020 Oct2026-05-03T10:11:13.115Z2025-04-04T19:50:17.122ZS-EPMC76486003299416410.1242/dev.195388