{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jacob JT"],"funding":["National Institutes of Health"],"pagination":["jcs254094"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7648610"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["133(20)"],"pubmed_abstract":["Keratin 17 (KRT17; K17), a non-lamin intermediate filament protein, was recently found to occur in the nucleus. We report here on K17-dependent differences in nuclear morphology, chromatin organization, and cell proliferation. Human tumor keratinocyte cell lines lacking K17 exhibit flatter nuclei relative to normal. Re-expression of wild-type K17, but not a mutant form lacking an intact nuclear localization signal (NLS), rescues nuclear morphology in KRT17-null cells. Analyses of primary cultures of skin keratinocytes from a mouse strain expressing K17 with a mutated NLS corroborated these findings. Proteomics screens identified K17-interacting nuclear proteins with known roles in gene expression, chromatin organization and RNA processing. Key histone modifications and LAP2β (an isoform en"],"journal":["Journal of cell science"],"pubmed_title":["Keratin 17 regulates nuclear morphology and chromatin organization."],"pmcid":["PMC7648610"],"funding_grant_id":["AR044232"],"pubmed_authors":["Matunis MJ","Coulombe PA","Jacob JT","Poll BG","Nair RR","Pineda CM","Hobbs RP"],"additional_accession":[]},"is_claimable":false,"name":"Keratin 17 regulates nuclear morphology and chromatin organization.","description":"Keratin 17 (KRT17; K17), a non-lamin intermediate filament protein, was recently found to occur in the nucleus. We report here on K17-dependent differences in nuclear morphology, chromatin organization, and cell proliferation. Human tumor keratinocyte cell lines lacking K17 exhibit flatter nuclei relative to normal. Re-expression of wild-type K17, but not a mutant form lacking an intact nuclear localization signal (NLS), rescues nuclear morphology in KRT17-null cells. Analyses of primary cultures of skin keratinocytes from a mouse strain expressing K17 with a mutated NLS corroborated these findings. Proteomics screens identified K17-interacting nuclear proteins with known roles in gene expression, chromatin organization and RNA processing. Key histone modifications and LAP2β (an isoform en","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-20T03:43:39.969Z","creation":"2025-04-20T03:43:39.969Z"},"accession":"S-EPMC7648610","cross_references":{"pubmed":["33008845"],"doi":["10.1242/jcs.254094"]}}