{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(5)"],"submitter":["Kapeleris J"],"pubmed_abstract":["<h4>Background</h4>Tumour tissue-based information is limited. Liquid biopsy can provide valuable real-time information through circulating tumour cells (CTCs). Profiling and expanding CTCs may provide avenues to study transient metastatic disease.<h4>Methods</h4>Seventy non-small cell lung cancer (NSCLC) patients were recruited. CTCs were enriched using the spiral microfluidic chip and a RosetteSep™ using bloods from NSCLC patients. CTC cultures were carried out using the Clevers media under hypoxic conditions. CTCs were characterized using immunofluorescence and mutation-specific antibodies for samples with known mutation profiles. Exome sequencing was used to characterized CTC cultures.<h4>Results</h4>CTCs (>2 cells) were detected in 38/70 (54.3%) of patients ranging from 0 to 385 CTCs "],"journal":["Translational lung cancer research"],"pagination":["1795-1809"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7653113"],"repository":["biostudies-literature"],"pubmed_title":["<i>Ex vivo</i> culture of circulating tumour cells derived from non-small cell lung cancer."],"pmcid":["PMC7653113"],"pubmed_authors":["Oleary C","Warkiani ME","Leo P","Punyadeera C","Kulasinghe A","Sternes P","O'Byrne K","Kapeleris J","Vela I"],"additional_accession":[]},"is_claimable":false,"name":"<i>Ex vivo</i> culture of circulating tumour cells derived from non-small cell lung cancer.","description":"<h4>Background</h4>Tumour tissue-based information is limited. Liquid biopsy can provide valuable real-time information through circulating tumour cells (CTCs). Profiling and expanding CTCs may provide avenues to study transient metastatic disease.<h4>Methods</h4>Seventy non-small cell lung cancer (NSCLC) patients were recruited. CTCs were enriched using the spiral microfluidic chip and a RosetteSep™ using bloods from NSCLC patients. CTC cultures were carried out using the Clevers media under hypoxic conditions. CTCs were characterized using immunofluorescence and mutation-specific antibodies for samples with known mutation profiles. Exome sequencing was used to characterized CTC cultures.<h4>Results</h4>CTCs (>2 cells) were detected in 38/70 (54.3%) of patients ranging from 0 to 385 CTCs ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-04T23:46:22.886Z","creation":"2025-04-04T23:46:22.886Z"},"accession":"S-EPMC7653113","cross_references":{"pubmed":["33209602"],"doi":["10.21037/tlcr-20-521"]}}