biostudies-literatureUnknown9(5)Kapeleris J<h4>Background</h4>Tumour tissue-based information is limited. Liquid biopsy can provide valuable real-time information through circulating tumour cells (CTCs). Profiling and expanding CTCs may provide avenues to study transient metastatic disease.<h4>Methods</h4>Seventy non-small cell lung cancer (NSCLC) patients were recruited. CTCs were enriched using the spiral microfluidic chip and a RosetteSep™ using bloods from NSCLC patients. CTC cultures were carried out using the Clevers media under hypoxic conditions. CTCs were characterized using immunofluorescence and mutation-specific antibodies for samples with known mutation profiles. Exome sequencing was used to characterized CTC cultures.<h4>Results</h4>CTCs (>2 cells) were detected in 38/70 (54.3%) of patients ranging from 0 to 385 CTCs Translational lung cancer research1795-1809https://www.ebi.ac.uk/biostudies/studies/S-EPMC7653113biostudies-literature<i>Ex vivo</i> culture of circulating tumour cells derived from non-small cell lung cancer.PMC7653113Oleary CWarkiani MELeo PPunyadeera CKulasinghe ASternes PO'Byrne KKapeleris JVela Ifalse<i>Ex vivo</i> culture of circulating tumour cells derived from non-small cell lung cancer.<h4>Background</h4>Tumour tissue-based information is limited. Liquid biopsy can provide valuable real-time information through circulating tumour cells (CTCs). Profiling and expanding CTCs may provide avenues to study transient metastatic disease.<h4>Methods</h4>Seventy non-small cell lung cancer (NSCLC) patients were recruited. CTCs were enriched using the spiral microfluidic chip and a RosetteSep™ using bloods from NSCLC patients. CTC cultures were carried out using the Clevers media under hypoxic conditions. CTCs were characterized using immunofluorescence and mutation-specific antibodies for samples with known mutation profiles. Exome sequencing was used to characterized CTC cultures.<h4>Results</h4>CTCs (>2 cells) were detected in 38/70 (54.3%) of patients ranging from 0 to 385 CTCs 2020-01-01T00:00:00Z2020 Oct2025-04-04T23:46:22.886Z2025-04-04T23:46:22.886ZS-EPMC76531133320960210.21037/tlcr-20-521