{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Babel H"],"funding":["Projekt DEAL","BASF"],"pubmed_abstract":["<h4>Background</h4>Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.<h4>Results</h4>Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → "],"journal":["Biotechnology for biofuels"],"pagination":["183"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7653855"],"repository":["biostudies-literature"],"pubmed_title":["Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol."],"pmcid":["PMC7653855"],"pubmed_authors":["Babel H","Kromer JO"],"additional_accession":[]},"is_claimable":false,"name":"Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol.","description":"<h4>Background</h4>Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.<h4>Results</h4>Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2026-05-08T20:14:11.489Z","creation":"2020-11-19T13:37:10Z"},"accession":"S-EPMC7653855","cross_references":{"pubmed":["33292484"],"doi":["10.1186/s13068-020-01825-6"]}}