biostudies-literatureUnknown13(1)Babel HProjekt DEALBASF<h4>Background</h4>Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.<h4>Results</h4>Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → Biotechnology for biofuels183https://www.ebi.ac.uk/biostudies/studies/S-EPMC7653855biostudies-literatureEvolutionary engineering of E. coli MG1655 for tolerance against isoprenol.PMC7653855Babel HKromer JOfalseEvolutionary engineering of E. coli MG1655 for tolerance against isoprenol.<h4>Background</h4>Isoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.<h4>Results</h4>Here we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → 2020-01-01T00:00:00Z2020 Nov2026-05-08T20:14:11.489Z2020-11-19T13:37:10ZS-EPMC76538553329248410.1186/s13068-020-01825-6