biostudies-literatureUnknown6(10)Bussotti GInstitut PasteurAlthough several studies have investigated genetic diversity of <i>Leishmania infantum</i> in North Africa, genome-wide analyses are lacking. Here, we conducted comparative analyses of nuclear and mitochondrial genomes of seven <i>L</i>. <i>infantum</i> isolates from Tunisia with the aim to gain insight into factors that drive genomic and phenotypic adaptation. Isolates were from cured (<i>n</i>=4) and recurrent (<i>n</i>=3) visceral leishmaniasis (VL) cases, originating from northern (<i>n</i>=2) and central (<i>n</i>=5) Tunisia, where respectively stable and emerging VL foci are observed. All isolates from relapsed patients were from Kairouan governorate (Centre); one showing resistance to the anti-leishmanial drug Meglumine antimoniate. Nuclear genome diversity of the isolates was analyMicrobial genomicshttps://www.ebi.ac.uk/biostudies/studies/S-EPMC7660250biostudies-literatureNuclear and mitochondrial genome sequencing of North-African <i>Leishmania infantum</i> isolates from cured and relapsed visceral leishmaniasis patients reveals variations correlating with geography and phenotype.PMC7660250Benkahla AJeddi FSpath GFSouiai OBouratbine ABussotti GAoun KfalseNuclear and mitochondrial genome sequencing of North-African <i>Leishmania infantum</i> isolates from cured and relapsed visceral leishmaniasis patients reveals variations correlating with geography and phenotype.Although several studies have investigated genetic diversity of <i>Leishmania infantum</i> in North Africa, genome-wide analyses are lacking. Here, we conducted comparative analyses of nuclear and mitochondrial genomes of seven <i>L</i>. <i>infantum</i> isolates from Tunisia with the aim to gain insight into factors that drive genomic and phenotypic adaptation. Isolates were from cured (<i>n</i>=4) and recurrent (<i>n</i>=3) visceral leishmaniasis (VL) cases, originating from northern (<i>n</i>=2) and central (<i>n</i>=5) Tunisia, where respectively stable and emerging VL foci are observed. All isolates from relapsed patients were from Kairouan governorate (Centre); one showing resistance to the anti-leishmanial drug Meglumine antimoniate. Nuclear genome diversity of the isolates was analy2020-01-01T00:00:00Z2020 Oct2026-05-02T19:18:05.555Z2020-11-19T17:00:20ZS-EPMC76602503297550310.1099/mgen.0.000444